Calcium and Bone Research
|Center of Calcium and Bone Research (COCAB)||Research Areas|
My research interest covers many aspects of calcium metabolism; from the mechanism of intestinal calcium absorption to the endocrine control of bone remodeling. We use multidisciplinary approach to study the mechanisms from systemic level to tissue, cellular and molecular levels.
Based on our previous investigations over the last two decades, we have shown that prolactin, the lactogenic hormone from the anterior pituitary gland, has an important role in the regulation of maternal calcium homeostasis. We demonstrated how prolactin induced increases in transcellular and paracellular intestinal calcium transport help prevent bone loss in lactation.
So far we have produced more than 25 international publications in support of our hypothesis of the role of prolactin as a Ca regulating hormone during growth, pregnancy and lactation.
We use a variety of techniques such as calcium balance, in situ intestinal sac perfusion in vitro electrophysiological and 45Ca kinetics studies and molecular technique to study the mechanism of intestinal calcium transport. We use measurements of bone density, bone histomorphometry, imaging techniques, biochemical markers of bone metabolism, and molecular studies of cultured primary bone cells and cell lines for elucidating mechanism of bone remodeling in physiological and pathological conditions.
With the use of atomic force microscopy and nanoindentation technique for determining the mechanical properties of bone, together with the study of bone microarchitecture, we are looking for indicators of early bone change in osteopenia. The knowledge might help prevent osteoporosis. Besides prevention, we also focus on treatment. We and our collaborators are designing hydroxyapatite nanocomposite with appropriate properties to be use as bone explants.
The Consortium for Calcium and Bone Research (COCAB) which is one of the multidisciplinary research units in the Center of Excellence of the Faculty of Science was established in 2003.
Members include academicians and researchers from the Faculty of Science and Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Srinakarinwiroj University, Chulalongkorn University, Kasetsart University and Thamasat University.
Our research activities range from physiological studies at systemic, organ, and cellular levels in experimental animals to biochemical molecular and genomic studies of cultured cells and clinical studies of the metabolic bone disorders.
Prof.Dr.Liangchai Limlomwongse (Senior Advisor)
Assoc. Prof .Dr. Narattaphol Charoenphandhu
Dr. Jantarima Charoenphandhu (Thammasat University)
Dr. Kannikar Wongdee (Burapha University)
Dr. La-iad Nakkrasae (Khonkaen University)
Dr. Weeraphat Pon-On (Kasetsart University)
Mrs. Wasana Saengamnart
Miss Jirawan Thongbunchoo
Miss Thitilux Leesakul
Miss Natchayaporn Thonapan
Miss Ratchaneevan Aeimlapa
Miss Phuntila Tharabenjasin
Miss Kanojwan Thongchote
Miss Nitita Dorkkam
Miss Panan Suntornsayatoon
Miss Pissared Khuituan
Miss Rattana Chaimara
Ms. Wacharaporn Tiyasatkulkovit
Mr. Sarawut Lapmanee
Miss Sirinthip Kamolsawat
Publications (Last 5 years)
1. Charoenphandhu N, Tudpor K, Thongchote K, Saengamnart W, Puntheeranurak S, Krishnamra N. High-calcium diet modulated effects of long-term prolactin exposure on the cortical bone calcium content in ovariectomized rats. Am J Physiol Endocrinology Metab. 2007; 292: E 443-52.
2. Anantamongkol U, Takemura H, Suthiphongchai T, Krishnamra N, Horio Y. Regulation of Ca2+ mobilization by prolactin in mammary gland cells: Possible role of secretory pathway Ca2+-ATPase type 2. Biochem Biophys Res Comm 2007; 352(2): 537-42.
3. Puntheeranurak S, Schreiber R, Spitzner M, Sausbier M, Ruth P, Krishnamra N, Kunzelmann K. Control of ion transport in mouse proximal and distal colon by prolactin. Cell Physiol Biochem 2007; 19: 77-88.
4. Charoenphandhu N, Wongdee K, Tudpor K, Pandaranandaka J, Krishnamra N. Chronic metabolic acidosis upregulated claudin mRNA expression in the duodenal enterocytes of female rats. Life Science 2007; 80: 1729-37.
5. Jantarajit W, Thongon N, Pandaranandaka J, Teerapornpuntakit J, Krishnamra N, Charoenphandhu N. Prolactin-stimulated transepithelial calcium transport in duodenum and Caco-2 monolayer are mediated by the phosphoinositide-3-kinase pathway. Am J Physiol Endocrinol Metab 2007; 293: E372-84.
6. Charoenphandhu N, Krishnamra N. Prolactin is an important regulator of intestinal calcium transport. Can J Physiol Pharmacol 2007; 85: 569-81.
7. Seriwatanachai D, Thongchote K, Charoenphandhu N, Pandaranandaka J, Tudpor K. Teerapornpuntakit J. Suthiphongchai T, Krishnamra N. Prolactin directly enhances bone turnover by raising osteoblast-expressed receptor activator of nuclear factor kB ligand/osteoprotegerin ratio. Bone 2008; 42: 535-46.
8. Thongchote K, Charoenphandhu N, Krishnamra N. High physiological prolactin induced by pituitary transplantation decreased BMD and BMC in the femoral metaphysis, but not in the diaphysis of adult female rats. J Physiol Sci 2008; 58: 39-45.
9. Charoenphandhu N, Teerapornpuntakit J, Methawasin M, Wongdee K, Thongchote K, Krishnamra N. Prolactin decreases expression of Runx2, osteoprotegerin, and RANK in primary osteoblasts derived from tibiae of adult female rats. Can J Physiol Pharmacol 2008; 86: 1-9.
10. Thongon N, Nakkrasae L, Thongbunchoo J, Krishnamra N, Charoenphandhu N. Prolactin stimulates transepithelial calcium transport and modulates paracellular permselectivity in Caco-2 monolayer: mediation by PKC and Rock pathways. Am J Physiol Cell Physiol 2008; 294: C1158-68.
11. Deachapunya C, Poonyachoti S, Krishnamra N. Regulation of electrolyte transport across cultured-endometrial epithelial cells by prolactin. J Endocrinol 2008; 197: 575-82.
12 Wongdee K, Pandaranandaka J, Teerapornpuntakit J, Tudpor K, Thongbunchoo J, Thongon N, Jantarajit W, Krishnamra N, Charoenphandhu N. Osteoblasts express claudins and tight junction-associated proteins. Histochem Cell Biol 2008; 130(1):79-90.
13. Seriwatanachai D, Charoenphandhu N, Suthiphongchai T, Krishnamra N. Prolactin decreases the expression ratio of receptor activator of nuclear factor kB-ligand/osteoprotegerin in human fetal osteoblast cells. Cell Biol Inter 2008; 32: 1126-35.
14. Tudpor K, Teerapornpuntakit J, Jantarajit W, Krishnamra N, Charoenphandhu N. 1,25-dihydroxyvitamin D3 rapidly stimulates the solvent drag-induced paracellular calcium transport in the duodenum of female rats. Physiol Sci 2008;58(5):297-307.
15. Charoenphandhu N, Wongdee K, Teerapornpuntakit J, Thongchote K, Krishnamra N. Transcriptome responses of duodenal epithelial cells to prolactin in pituitary-grafted rats. Molec Cell Endocrinol 2008; 296: 41-52.
16. Seriwatanachai D, Krishnamra N, van Leeuwen JPTM. Evidence for direct effects of prolactin on human osteoblasts: inhibition of cell growth and mineralization. J Cell Biochem 2009; 107: 677-85.
17. Wongdee K, Teerapornpuntakit J, Riengrojpitak S, Krishnamra N, Charoenphandhu N. Gene expression profile of duodenal epithelial cells in response to chronic metabolic acidosis. Molec Cell Biochem 2009; 321(1-2): 173-88.
18. Teerapornpuntakit J, Dorkkam N, Wongdee K, Krishnamra N, Charoenphandhu N. Endurance swimming stimulates transepithelial calcium transport and alters the expression of genes related to calcium absorption in the intestine of rats. Am J Physiol Endocrinol Metab 2009; 296(4): E775-86.
19. Thongon N, Nakkrasae L, Thongbunchoo J, Krishnamra N, Charoenphandhu N. Enhancement of calcium transport in Caco-2 monolayer through PKCζ-dependent Cav1.3-mediated transcellular and rectifying paracellular pathways by prolactin. Am J Physiol Cell Physiol 2009; 296(6): C1373-82.
20. Kraidith K, Jantarajit W, Teerapornpuntakit J, Nakkrasae L, Krishnamra N, Charoenphandhu N. Direct stimulation of the transcellular and paracellular calcium transport in the rat cecum by prolactin. Pflügers Archiv - Eur of J Physiol 2009; 458(5): 993-1005.
21. Assapun J, Charoenphandhu N, Krishnamra N. Early acceleration phase and late stationary phase of remodeling imbalance in long bones of male rats exposed to long-standing acidemia: A 10-month longitudinal study using bone histomorphometry. Calcif Tiss Inter 2009; 85(1): 1-9.
22. Charoenphandhu N, Nakkrasae L, Kraidith K, Teerapornpuntakit J, Thongchote K, Thongon N, Krishnamra N. Two-step stimulation of intestinal Ca2+ absorption during lactation by long-term prolactin exposure and suckling-induced prolactin surge. Am J Physiol Endocrinol Metab 2009; 297(3): E609-19.
23. Domrongkitchaiporn S, Disthabanchong S, Cheawchanthanakij R, Niticharoenpong K, Stitchantrakul W, Charoenphandhu N, Krishnamra N. Oral phosphate supplementation corrects hypophosphatemia and normalizes plasma FGF23 and 25-hydroxyvitamin D3 levels in women with chronic metabolic acidosis. Explt Clin Endocrinol Diabetes 2010; 118: 105-12.
24. Pusoonthorntum R, Pusoonthorntum P, Krishnamra N. Calcium-phosphorus homeostasis and changes in parathyroid hormone secretion in cats with various stages of spontaneous chronic renal failure. Comp Clin Pathol 2010; 19: 287-93.
25. Suntornsaratoon P, Wongdee K, Krishnamra N, Charoenphandhu N. Femoral bone mineral density and bone mineral content in bromocriptine-treated pregnant and lactating rats. Physiol Sci 2010; 60(1): 1-8.
26. Nuntapornsak A, Wongdee K, Thongbunchoo J, Krishnamra N, Charoenphandhu N. Changes in the mRNA expression of osteoblast-related genes in response to β3-adrenergic agonist in UMR106 cells. Cell Biochem Func 2010; 28(1): 45-51.
27. Nakkrasae L, Thongon N, Thongbunchoo J, Krishnamra N, Charoenphandhu N. Transepithelial calcium transport in prolactin-exposed intestine-like Caco-2 monolayer after combinatorial knockdown of TRPV5, TRPV6 and Cav1.3.
28. Anantamongkol U, Charoenphandhu N, Wongdee K, Teerapornpuntakit J, Suthiphongchai T, Prapong S, Krishnamra N. Transcriptome analysis of mammary tissues reveals complex patterns of transporter gene expression during pregnancy and lactation. Cell Biol Inter 2010; 34(1): 67-74.
29. Wongdee K, Riengrojpitak S, Krishnamra N, Charoenphandhu N. Claudin expression in the bone-lining cells of female rats exposed to long-standing acidemia. Explt Molec Pathol 2010; 88(2): 305-10.
30. Charoenphandhu N, Wongdee K, Krishnamra N. Is prolactin the cardinal calciotropic hormone in mothers? Trends Endocrinol Metabol 2010; 7: 395-401.
31. Suntornsaratoon P, Wongdee K, Goswami S, Krishnamra N, Charoenphandhu N. Bone modeling in bromocriptine-treated pregnant and lactating rats: possible osteoregulatory role of prolactin in lactation. Am J Physiol Endocrinol Metab 2010 (in press).
32. Tang IM, Krishnamra N, Charoenphandhu N, Hoonsawat R, Pon-On W. Biomagnetic of apatite-coated cobalt ferrite: a core-shell particles for protein adsorption and pH controlled release. Nano Res Letters 2010 (in press).
33. Jongwattanapisan P, Charoenphandhu N, Krishnamra N, Thongbunchoo, Tong I-M, Hoonsawat R, Pon-On W. Invitro study of the SBF and osteoblast –like cells on hydroxyapatite/chitosan-silica nanocomposite. J Mat Sci Eng 2010
34. Suntornsaratoon P, Wongdee K, Krishnamra N, Charoenphandhu N. Possible chondroregulatory role of prolactin as indicated by changes in tibial growth plate in bromocriptine treated pregnant and lactating rats. Histochem Cell Biol 2010 (in press)
35. Wongdee K, Dulalamba W, Thongbunchoo J, Krishnamra N, Charoenphandhu N.Prolactin alters the mRNA expression of osteoblast-derived osteoclastogenic factors in osteoblast-like UMR106 cells. (in press)