The Third International Symposium on
  Plant Cryopreservation (CryoSymp 2018)

March 26-28, 2018
Bangkok, Thailand
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Invited Speakers

Dr. Ir. Bart Panis

"60 years of plant cryopreservation: from freezing hardy mulberry twigs to establishing reference crop collections for future generations".

Senior Researcher, Bioversity International, Leuven, Belgium

      Bart Panis started his career in 1989 as a research assistant at the University Leuven, Belgium, where he was involved in cryopreservation and the development of embryogenic cell suspensions, protoplast culture and techniques for genetic engineering of banana. In 1994, he co-developed the world's first transgenic banana. He obtained his PhD degree in 1995 on cryopreservation of Musa spp. As postdoctoral researcher and later on as research manager, he co-ordinated many international projects dealing with plant biotechnology and cryopreservation. A major achievement is the development of the world's largest banana cryo-bank at the Bioversity International Musa Transit Centre. In 2013, he started working for Bioversity International.

Bart Panis is a member of the editorial board of CryoLetters and Associate Editor of Plant Cell Tissue and Organ Culture and organized 12 international Symposia and workshops. Moreover, he was actively involved (as chairman, co-organizer or invited speaker) in the organization of about 30 other International congresses. He is board member of the SLTB (Society for Low Temperature Biology, London), Council member for Belgium and Vice-chair of the Commission of biotechnology of the ISHS (International Society for Horticultural Science). His scientific results were written down in 350 articles of which more than 100 are in international refereed journals (Web of science), resulting in an h-factor of 24.

Prof. Dr. Maurizio Lambardi

"Cryopreservation of temperate fruit trees: a critical evaluation of practical applications after 30 years of experimentation"

Senior Researcher, National Research Council (CNR) of Italy, IVALSA/Trees and Timber Institute, Florence, Italy

Maurizio LAMBARDI is Senior Researcher of the National Research Council (CNR) of Italy at the IVALSA Institute, and appointed Full Professor in the sector Arboriculture and Forest Systems. For the ISHS (International Society for Horticultural Science), he is Chairperson of the Commission 'Molecular Biology and In Vitro Culture', and co-representitive for Italy of the Council Board. He is also Academic Correspondent of the Italian 'Accademia dei Georgofili', General Secretary of the Italian Society of Horticulture (SOI), Coordinator of the Italian Working Group on 'Micropropagation and In Vitro Technologies, and Honorific Member of the SHST/Horticulture and Forestry Society from Transylvania. He was the former Chairman of the Society for Low Temperature Biology (SLTB). He is member of the Editorial Board of numerous International ISI journals, among which Plant Cell Tissue and Organ Culture, Acta Physiologiae Plantarum, CryoLetters, European Journal of Horticultural Science, Propagation of Ornamental Plants, Ciencia e Agrotecnologia, Notulae Botanicae Horti Agrobotanici Cluj-Napoca. He has a large expertise in the development of advanced in vitro technologies, and their application to the propagation and preservation of woody plant genetic resources. In particular, he contributed to the exploitation of cryopreservation techniques for the long-term conservation of tissues and organs from fruit and forest species. He is author or co-author of over 170 full-length papers and co-editor of international books on micropropagation and tissue culture.

Professor Dr. Hugh Pritchard

"Thermal fingerprinting and seed deterioration at low temperatures"   Hugh W. Pritchard, Jayanthi Nadarajan, Louise Colville and Dani Ballesteros Royal Botanic Gardens, Kew, UK

Head of Comparative Seed Biology, Royal Botanic Gardens (RBG) Kew, United Kingdom

Hugh Pritchard has a PhD in plant cryobiology and >30 years' experience in genetic resources preservation, including as a member of the senior management team delivering the Millennium Seed Bank Project / Partnership through to 2014. His research specialities include seed cryopreservation, germination modelling and stress biology. He has published >200 scientific papers (c. 55% in international peer-reviewed journals), including in the Proceedings of the National Academy of Sciences, The Plant Journal, Biotechnology Advances and Trends in Plant Science. His Google Scholar h-index is 40. His research work is multidisciplinary in approach and his research group (8 post-doctoral fellows, supporting 16 PhD students currently) has global connections, publishing with co-authors from >30 countries (from Brazil to China) in the last 10 years. He has led four Darwin Initiative (UK) projects (2003-17) involving 16 countries in Africa, 9 countries in the Americas and 6 countries in Asia. He has managed Kew's role on two EU Framework 7 projects on 'climate and seed quality' and 'native seed biology' and a NERC project on seeds of Amazonian plants. In addition to being a publisher of the low temperature science journal CryoLetters, he has been chairman of the Society for Low Temperature Biology (2008-11) and a governor at Writtle University College, UK (2008-12). He is chairman of the Seed Storage Committee of the International Seed Testing Association. He holds honorary professorships from the University of Sussex and the Chinese Academy of Sciences, is a fellow of the Royal Society of Biology and the Linnean Society; and is an elected member of the Academy of Sciences of South Africa. He was a Senior International Visiting Professor with the Chinese Academy of Sciences in 2011 and 2017. He leads for Kew on the collaboration with the Indian Council of Agricultural Research and the Chinese Academy of Science.

Examples of recent publications on seed desiccation and cryobiotechnology

Chen H-Y, Yu X-M, Pritchard HW, Li W-Q (2017). Phospholipase Dα1-mediated phosphatidic acid production is a key determinant of desiccation-induced viability loss in seeds. Plant Cell & Environment

Han E-J, Popova E, Cho G-T, Park S-U, Lee S-C, Pritchard HW, Kim H-H. (2016). Post-harvest embryo development in ginseng seed increases desiccation sensitivity and narrows the hydration window for cryopreservation. CryoLetters 37, 284-294.

Malik SK, Chaudhury R, Pritchard HW (2012) Long-term, large-scale banking of Citrus species embryos: comparisons between cryopreservation and other seed banking temperatures.  CryoLetters 33, 219-230. 

Nadarajan J, Pritchard HW (2014). Biophysical characteristics of successful oilseed embryo cryoprotection and cryopreservation using vacuum infiltration vitrification, an innovation in plant cell preservation. PLoS ONE online 1 May 2014, DOI: 10.1371/journal.pone.0096169.

Popova E, Sylvestre I, Kim H-H, Kumar Saxena P, Engelmann F, Pritchard HW (2016). Frozen beauty: the cryobiotechnology of orchid diversity. Biotechnology Advances 34, 380-403

Pritchard HW, Moat J, Ferraz JBS, Marks TR, Camargo JLC, Nadarajan J, Ferraz IDK (2014). Innovative approaches to the preservation of tree species. Forest Ecology & Management 33, 88-98. 

Pritchard HW (2016). Priority science for the preservation of priority crops. Indian Journal of Plant Genetic Resources 29, 292-297. DOI 10.5958/0976-1926.2016.00049.8

Wade EM, Nadarajan J, Yang X-Y, Ballesteros D, Sun W-B, Pritchard HW (2016). Plant species with extremely small populations (PSESP) in China: a seed and spore biology perspective. Plant Diversity 38, 209–220.

Dr. Takao Niino

"Development V and D cryo-plate methods as effective protocols for cryobanks"

Senior Scientist of JICA/JST SATREPS "Diversity Assessment and Development of Sustainable Use of Mexican Genetic Resources), Gene Research Center, Univ. Tsukuba, Tsukuba, Japan

Takao Niino started his career of plant cryopreservation in 1988 as a researcher at the Research Institute, Shinjo, Japan where he was involved in cryopreservation of dormant buds and establishment of in vitro shoots of woody plants, such as mulberry, pear and apple. Around 2000, he developed cryopreservation protocols by vitrification and encapsulation-dehydration with Prof. Dr. Akira Sakai using strawberry, Innala, leek and chrysanthemum. From 2003 to 2006, as a senior researcher of the Genebank, National Institute of Agrobiological Sciences (NIAS), Tsukuba, Japan, he contributed construction of NIAS-Cryo bank of mulberry dormant buds and in vitro mat rush and strawberry shoot tips based on his career. From 2009 to 2012, as a senior scientist of the Genebank, he started to develop a new technique using an aluminium cryo-plate, resulting successful cryopreservation of several plants with high regeneration by V and D cryo-plate methods. In 2012, he started working for JICA/JST SATREPS project "Diversity Assessment and Development of Sustainable Use of Mexican Genetic Resources" as a senior scientist, Univ. Tsukuba, Tsukuba, Japan. He has applied both methods to Mexican original plants such as potato, chayote and vanilla. By these contributions, his group obtained "Technical Award of Japanese Society for Plant Cell and Molecular Biology" twice in 2003 and 2016.


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