Lect. Nisamanee Charoenchon, Ph.D.   
Department of Pathobiology,
Faculty of Science, Mahidol University
272 Rama VI Road, Phayathai
Ratchathewi, Bangkok 10400, THAILAND
Tel: 662-201-5573
Fax: 662-354-7158
<<<PDF File>>>     last update : April 28, 2017



I am Nisamanee Charoenchon (BSc (Hons), MSc), awarded PhD degree in Medicine program by research from the
University of Manchester, UK and funded by DPST scholarship, the Royal Thai Government since 2002.



2012-2016 • PhD, Doctor of Philosophy (Medicine), Degree approval date: 16th Nov, 2016,
                   School of Biological Sciences, Faculty of Biology, Medicine & Health
                   The University of Manchester, United Kingdom

2009- 2012 • Master of Science (Biotechnology), GPA : 3.83, Chulalongkorn University, Bangkok, Thailand

2005- 2009 • Bachelor of Science (Biology), first class honors, GPA: 3.62, Khon Kaen University, Khon Kaen, Thailand


The recent topics and significance

My recent studies were related to monitoring and assessment the histological alteration in collagen and elastic fibre
system in papillary dermis in vivo under the condition of concentrate solar simulation radiation (SSR) and UVB. The field
is also included the supplementation, green tea catachin extract which may help those extra cellular matrix repairing
in case of damaging occur due to acute SSR.

Specifically the objective in the part of collagen study is to estimate fibrillar collagen remodelling and remaining pro-collagenI
in fifty healthy subjects at baseline and after irradiated with SSR before evaluate the outcome in these matrices. Besides
the study would be processed concurrently with a randomised controlled trial of green tea catechin (GTC) supplementation
in pre- and post- supplementation.

The skin tissue from volunteers were performed through histochemical stain techniques and continued with image analysis
and semi-quantitative analysis. This is a pilot study in vivo in human of the effect SSR, imitated the natural light on ECM
distribution in dermal skin along with the impact of high dose catechins to repair them if ECM damage occurs. The results
may reflect phenomenal of ECM reorganization in daily life due to UV irradiation from the sun and if catechin supplement
shows capability of skin protection particularly in major ECM, such as fibrillar collagen, this will be the interesting choice for
the customer to choose the most convenient way to protect against skin damage from UV.


Research Experiences

2012- present Thesis topic “Can green tea catechins protect against photoageing?
supervised by Dr. Rachel Watson | Division of Musculoskeletal & Dermatological Sciences | School of Biological Sciences |
Faculty of Biology, Medicine & Health | The University of Manchester
E-mail :

- Histochemical and immunohistochemical staining
- Human tissue frozen section (cryosectioing technique)
- Image analysis by Image J

2009- 2012 Thesis topic “Development of DNA sensor for Crustacean Allergen Analysis in Food Products
supervised by Asst. Prof. Piyasak Chaumpluk | Department of Botany | Faculty of Science | Chulalongkorn university

- Primer design
- Gene cloning
- DNA amplification by using PCR and isothermal technique
- Using fluorescence beta-pyrroridinyl peptide nucleic acid probe as bio-sensor in system

2008- 2009 Senior project topic of BSc “Protein Expression in Leaves of Sugarcane (Saccharum spp.) under Water Stress



(1) Poster Presentation: Dietary green tea catechins protect dermal elastic fibers from UV-induced remodeling. Society For
Investigative Dermatology (SID2016), May 11th-14th, 2016, Scottsdale, US

(2) Poster Presentation: The impact of oral green tea catechin supplementation
on the collagenous matrix following acute irradiation. British Society for Investigative Dermatology (BSID2016), April 4th-6th,
2016, Dundee, UK

(3) Poster Presentation: The effect of acute solar-simulated radiation (SSR) on the distribution of dermal elastic fibres. European
Society for Dermatological Research (ESDR2014), September 10th-14th, 2014, Copenhagen, Denmark

(4) Oral Presentation : Simple DNA-on-a-Chip System for Detection of Crustacean Allergenic Residues in Food Matrix. Pure and
Applied Chemistry International Conference (PACCON2012), January 11th-13th, 2012, Chiang Mai, Thailand.

(5) Poster Presentation : Development of DNA sensor for Crustacean Allergen Analysis in Food Products. Food Allergy and
Anaphylaxis Meeting (FAAM2011), February 17th-19th, 2011, Venice, Italy.

(6) Oral Presentation : Development of DNA Based Biosensor for
Crustacean Allergen Related Constituents Analysis. Pure and Applied Chemistry International Conference (PACCON2011), January
5th -7th, 2011, Bangkok, Thailand.


International Publications

(1) Proceeding. Simple DNA-on-a-chip system for detection of Crustacean allergenic residues in food matrix. Pure and Applied
Chemistry International Conference (PACCON2012) January 11th-13th, 2012, Chiang Mai, Thailand.



Department of Pathobiology, Faculty of Science, Mahidol University,
  Rama VI Road, Bangkok 10400, Thailand.
  Phone : (662) 201-5550, 354-7158  Fax : (662) 354-7158 
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