Dr. Watanalai  Panbangred

 Group : Molecular and Medical Biotechnology

 

    MU-OU
 

Contact Details :

 

Department of Biotechnology, Faculty of Science, Mahidol University
Rama VI Road, Bangkok 10400, Thailand
Tel : 0-2201-5927 Fax : 0-2201-5926
E-mail :
watanalai.pan@mahidol.ac.th

 

Current Academic Position :

 

Professor, Department of Biotechnology, Faculty of Science, Mahidol University

 

Research Interest :

 

Development of PCR kit for rapid detection of Salmonella and Bacillus cereus in foods.
Screening of Streptomyces and characterization of their metabolites

 

Education :

 

1971-1975

B.Sc.(Biology), Mahidol University, Thailand

 

1975-1978

M.Sc. (Microbiology), Mahidol University, Thailand

 

1978-1979

Diploma (Microbial Technology), Osaka University, Japan

 

1980-1984

Dr.Eng.(Fermentation Technology), Osaka University, Japan

 

1990-1991

Post doctoral fellow, The Salk Institue, San Diego, California, USA

 

Fellowships and Awards :

 

1978-1979

UNESCO Fellowship

 

1980-1984

Monbusho Scholarship

 

1990

Taguchi Prize :Outstanding Young Scientist in Biotechnology

 

1990-1991

Rockefeller Fellowship

 

1992

IRRI Award : Outstanding Yong Woman in Rice Sciences

 

2005

Best Teacher Award from Senate Council, Mahidol University

 

2006

Best Teacher in Science and Technology Award, Council of the University Faculty Senate of Thailand (CUFST)

 

Professional experience :

 

August 14, 1984

Lecturer Dept. of Microbiology, Faculty of Science, Mahidol University

 

August 28, 1986

Assistant Professor, Dept. of Microbiology, Faculty of Science, Mahidol University

 

September 30, 1990

Associate Professor, Dept. of Biotechnology, Faculty of Science, Mahidol University

 

April 15,1990 –July 30, 1991

Postdoctoral Fellow, The Salk Institute, San Diego, CA, USA

 

January 20, 1994 – Jan 19, 1998

Head, Dept. of Biotechnology, Faculty of Science, Mahidol University

 

April 1996 – March 1998

Assistant co-ordinator : Graduate Research Eduction Consortium, National Science and Technology Development Agency

 

July 1998 – December 1998

Visiting Professor, Osaka University, Japan

 

January 1999 – September 2002

Deputy Dean, Faculty of Graduate Studies, Mahidol University

 

August 30, 2002

Professor, Dept. of Biotechnology, Faculty of Science, Mahidol University

 

December 2002 - present

Co-ordinator : Mahidol University-Osaka University Collaborative Research Center for Bioscience and Biotechnology (MU – OU : CRC)


Patent :

1. Panbangred, W. and Honda, J. (2008)  Method of producing palatinose, trehalulose or mixture thereof. Patent Number JP2008079533A, Japan.
2. Petre, J., Boonchird, C., Buasri, W. and Panbangred, W. (2011)  Improved Bordetella pertussis strains for the production of recombinant acellular pertussis vaccine. Patent Number 2011/09417., South Africa.

 

Publications :

1.   Panbangred, W.,Bhumiratana, A. and Pantuwatana, S. (1979). Toxicity of Bacillus thuringiensis toward Aedes aegypti larvae. J. Invertebr. Pathol. 33 : 340-347.

 

2.   Panbangred, W., Shinmyo, A., Kinoshita, S. and Okada, H. (1982). Purification and properties  of endoxylanase produced by Bacillus pumilus. Agri. Biol. Chem. 47: 957-963.     

The Journal’s name was changed to Bioscience, Biotechnology and Biochemistry.
 

3.   Panbangred, W., Kondo T., Negoro T., Shinmyo A. and Okada, H. 1983. Molec cloning of the genes for xylan degradation of Bacillus pumilus and their expression in Escherichia coli. Mol. Gen. Genet. 192 : 335-341.           
 

4.   Panbangred, W., Kawaguchi, O., Tomita, T., Shinmyo, A. and Okada, H. (1984). Isolation of two  b-xylosidase genes of Bacillus pumilus and comparison of their gene products. Eur. J. Biochem. 138 : 267-273.

 

5.   Fukusaki, E., Panbangred, W., Shinmyo, A., and Okada, H.(1984). The complete nucleotide sequence of the xylanase gene (xyn A) of Bacillus pumilus. FEBS Lett. 171 : 197-201.

 

6.   Panbangred, W., Fukusaki, E., Epifanio, E.C., Shinmyo, A. and Okada,H. (1985). Expression of a xylanase gene of Bacillus pumilus in Escherichia coli and Bacillus subtilis. Appl. Microbiol. Biotechnol. 22 : 259-264.

 

7.   Panbangred, W., Pilantanapak, A., Jayanetra, P. and Bhumiratana, A. (1987). R plasmid confering multiple drug resistance from Salmonella krefeld isolated in Thailand. Microbial Utilization of Renewable Resources. 5 : 42-49.

 

8.   Panbangred, W., Udombunditkul, M. and Meevootisom, V. (1989). High expression of penicillin acylase gene in genetically engineered Escherichia coli. Microbial Utilization of Renewable Resources. 7 : 404-413

 

9.  Panbangred, W. (1990). Genetic engineering : From bacteria to rice gene. Microbial Utilization of Renewable Resources. 8 : 144-157.

 

10. Panbangred, W., Varavinit, S., Vichitsoonthonkul, T., Intararuangsorn, S. and Meevootisom, V. (1990) Research and development of a biotechnological process for 6 Amino-penicillanic acid production. Microbial Utilization of Renewable Resources 8 : 247-253.

 

11. Pilantanapak, A., Jayanetra, P., Bhumiratana, A. and Panbangred,W., (1990). Biotinylated drug resistant probes for Salmonella krefeld. J. Antimicrob  Chemother.  25 : 593-603.

 

12. Wiwat, C., Panbangred, W., and Bhumiratana, A. (1990) Transfer of plasmids and chromosomal genes amongst subspecies of Bacillus thuringiensis. J. Indust. Microbiol. & Biotech. 6 : 19-27.

 

13. Trisrisook, M., Pantuwatana, S., Bhumiratana, A., and Panbangred, W., (1990) Molecular cloning of the 130-kilodalton mosquitocidal  d - endotoxin gene of Bacillus thuringiensis subsp.israelensis in Bacillus sphaericus. Appl. Environ. Microbiol. 56 : 1710-1716.

 

14. Panbangred, W., Jayanetra, P. and Pilantanapak, A. (1990). Epidemiological study of sulfonamide and trimethoprim resistance genes in enterobacteriaceae. Southeast Asian J. Trop. Med. Public Health 21 : 175-184.

15. Jayanetra, P., Vorachit, M., Pilantanapak, A., Panbangred, W., Bangtragulnonth, A. and Pan-urai, R.(1990). Salmonella krefeld in Thailand: I. Epidemiology, infection and drug resistance. Southeast Asian J. Trop. Med. Public Health 21 : 354-360.

 

16. Jayanetra, P., Vorachit, M., Pilantanapak, A., Panbangred, W., Bangtragulnonth, A. and Pan-urai, R. (1990). Salmonella krefeld in Thailand : II Molecular biology of drug resistance. Southeast Asian J. Trop. Med. Public Health. 21 : 361-366.

 

17. Panbangred, W., Udomvaraphunt, S., and Meevootisom,V.(1990)  Genetic manipulation of penicillin acylase in Bacillus sp. J. Sci. Soc. of Thailand 16 : 55-75.

 

18. Tantipaiboonwat, S., Panbangred, W., Bhumiratana, A., and Pantuwatana, S. (1990) Selection of insecticidal activity of Bacillus thuringensis subsp. kurstaki. Microbial Utilization of Renewable Resources 7 : 283-286.

 

19. Wiwat, C., Panbangred, W., Pantuwatana, S., Mongkolsuk, S., and Bhumiratana, A. (1992). Molecular cloning of S-layer protein gene from Bacillus thuringiensis. In “Biotechnology and Environmental Science : Molecular Approaches” S. Mongkolsuk, P.S. Lovett, and J.E. Trempy (eds). Plenum Press, U.S.A.

 

20. Palittapongarnpin, P. Rienthong, S. and Panbangred, W. (1993). Comparison of restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from cerebrospinal fluid and sputum : A preliminary report. Tubercle and Lung Diseases 74:204-207.

 

21. De-Eknamkul, C. and Panbangred W., (1993) Bacterial genetics. In Basic Bacteriology. (In Thai) 1st ed. Supavet, S. and Vorachit, M. (eds)., Siriyod Print Co., Ltd., Bangkok, Thailand, pp 72-141.

 

22. Panbangred W., and Udomvaraphant, S. (eds) Manual of Biotechnology : Molecular Genetics and Genetic Engineering Techniques. (in Thai) 1st ed. Thai Society for Biotechnology. 733 p. 1993.

 

23. Bhumiratana A., Panbangred, W., Thepsingha, W., and Wiwat, C. (1994). Research and development for microbial based biopesticides in Thailand. International Workshop on Application and Control of Microorganism in Asia. Tokyo. pp. 23-32.

 

24. Wiwat, C., Panbangred, W., Mongkolsuk, S., Pantuwatana, S., and Bhumiratana, A. (1995). Inhibition of a conjugation-like gene transfer process in Bacillus thuringiensis subsp. israelensis by the anti-S-layer protein antibody. Current Microbiol. 30 : 69-75.

 

25. Xu, Y; Zhu, Q., Panbangred, W., Shirasu, K. and Lamb, C. (1996) Regulation expression and a function of a new basic chitinase gene in rice (Oryza sativa L.). Plant Mol. Biol. 30 : 387-401.

 

26. Lappayawichit, P., Rienthong, S., Rienthong, D., Chuchottaworn, C., Chaiprasert, A., Panbangred, W., Saringearinkul, H. and Palittapongarnpim, P. (1996) Differentiation of Mycobacterium species by restriction enzyme analysis of amplified 16S-23S ribosomal DNA spacer sequences. Tubercle and Lung Disease. 77 : 257-263.

 

27. Sirichotpakorn, N., Panbangred, W., Pantuwatana, S. and Bhumiratana, A. (1996). The effect of cryIVB terminator fragments on expression of the chloramphenicol acetyltransferase gene in Bacillus thuringiensis subsp. israelensis. J. Sci. Soc. Thailand 22 : 121-130.

 

28. Panjaisri, S., Charoenpornwatana, S., Pantuwatana, S., Bhumiratana, A. and Panbangred, W. (1997) High production of CryIVB protein does not lead to formation of crystalline inclusions in Bacillus thuringiensis susp. israelensis. World J. Microbiol & Biotechnol. 13:319-327.

 

29. Sriubolmas, N., Panbangred, W., Sriurairatana, S. and Meevootisom, V. (1997) Localization and characterization of inclusion bodies in recombinant Escherichia coli cells overproducing penicillin G acylase. Appl. Microbiol. Biotechnol. 47:373-378.

 

30. Pilantanapak, A., Jayanetra, P., Panbangred, W., Klungthong, C. and Bangtrakulnonth, A. (1997). Construction of specific DNA probe for the detection of Salmonella in food. Southeast Asian J. Trop. Med. Public Health. 28:73-81.

 

31. Klungthong, C., Pilantanapak, A., Jayanetra, P. and Panbangred, W. (1997). Detection of Escherichia coli by DNA hybridization and polymerase chain reaction. Asia Pacific J. Mol. Biol. Biotech. 5:98-110.

 

32. Tantimavanich, S., Pantuwatana, S., Bhumiratana, A. and Panbangred, W. (1997) Cloning of a chitinase gene into Bacillus thuringiensis subsp. aizawai for enhanced insecticidal activity. J. Gen. Appl. Microbiol. 43: 341-347.

 

33. Tantimavanich, S., Pantuwatana, S., Bhumiratana, A. and Panbangred, W. (1998) Multiple chitinase enzymes from a single gene of Bacillus licheniformis TP-1.                J. Biosc  Bioeng. 85: 259-265.

 

34. Charoenpornwatana, S., Thara, K.V., Wang, L., Datta, S.K., Panbangred, W. and Muthukrishnan, S. (1999) Inheritance, expression, and silencing of a chitinase transgene in rice. Theor, Appl. Genet. 98:371-378.

 

35. Tapchaisri, P., Wangroongsarb, P., Panbangred, W., Kalambaheti, T., Chongsa-nguan, M., Srimanote, S., Kurasano, H., Hayashi, H. and Chaicunpa. (1999) Detection of Salmonella contamination in food samples by dot-ELISA, DNA amplification and facterial culture. Asian Pacific J. Allergy and Immand. 17: 41-51.

 

 36. Panbangred, W., Panjaisee, S., and Tantimavanich, S. (2000). Expression of the mosquitocidal cryIVB gene under the control of different promoters in Bacillus sphaericus 2362 and acrystalliferous Bacillus thuringiensis subsp. israelensis c4Q2-72.  World J. Microbiol. & Biotechnol. 16: 163-169.

 

37. Panbangred, W., Weeradechapon, K., Udomvaraphant, S., Fujiyama, K., and Meevootisom, V. (2000). High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant.  J. Appl. Microbiol. 89: 158-168.

 

38. Thamthiankul, S., Suan-Ngay, S., Tantimavanich, S. and Panbangred , W. (2001).  Chitinase from Bacillus thuringiensis subsp. pakistani. Appl. Microbiol. Biotechnol. 56:395-401.

 

39. Sirichotpakorn, N., Rongnoparut, P., Choosang. K. and Panbangred, W. (2001). Coexprssion of chitinase and the cry11Aal toxin gene in Bacillus thuringiensis subsp. israelensis. J. Invertebr. Pathol. 78 : 160-169.

 

40. Aunpad, R., Muench, S.P., Baker, P.J., Sedenikova, S., and Panbangred, W. Doukyu, N., Aono, R. and Rice, D.W. (2002). Crystallization and preliminary X-ray crystallography studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin.  Acta Cryst (Section D). 58:2182-2183.

 

41. Aunpad, R. and Panbangred, W. (2003) Cloning and characterization of the constitutively expressed chitinase C gene from a marine bacterium, Salinivibrio costicola 5SM-1. J. Biosc. Bioeng. 96:529-536.

 

42. Thamthiankul, S., Moar, W.J., Miller M., and Pangbangred, W. (2004) Improving the insecticidal activity of Bacillus thuringiensis subsp. aizawai against Spodoptera exigua by chromosomal expression of a chitinase gene. Appl. Microbiol. Biotechnol. 65:183-192.

 

43. Wang, X., Upatham, S., Pangbangred, W., Isarangkul, D., Summpunn, P., Wiyakrutta, S., and Meevootisom, V. (2004)  Purification, characterization, gene cloning and sequence analysis of a phytase from Klebsiella pneumoniae subsp. pneumoniae XY-5.  ScienceAsia 30:383-390.

 

44. Thaenthanee, S., Wong, A.C., Panbangred W. (2005)  Phenotypic and genotypic comparisons reveal a broad distribution and heterogeneity of hemolysin BL genes among Bacillus cereus isolates.  Int. J. Food Microbiol. 105:203-212.

 

45. Aroonnual A,., Nihira T., Seki, T. and Panbangred, W. (2007)  Role of several key residues in the catalytic activity of sucrose isomerase from Klebsiella pneumoniae.  Enz.  Microb. Tech. 40: 1221-1227.

 

46. Aunpad, R., Rice, D.W., S. Sedelnikova, D. and Panbangred, W. (2007)  Biochemical characterization of two-forms of halo-and thermo-tolerant chitinase C of Salinivibrio costicola expressed in E. coli.  Annals Microbiol. 57(2):249-257.

 

47. Suwanjinda, D., Eames, C. and Panbangred, W. (2007)  Screening of lactic acid bacteria for bacteriocins by microbiological and PCR methods.  Biochem. Mol. Biol. Educ. 35:364-369.

 

48. Ngamwongsatit, P., Buasri, W., Pianariganon, P., Pulsrikarn, C., Ohba, M., Assavanig, A. and Panbangred, W. (2008)  Broad distribution of enterotoxin genes (hblCDA, nheABC, cytK and entFM) among Bacillus thuringiensis and Bacillus cereus as shown by novel primers. Int. J. Food Microbiol. 121:352-356.

 

49. Ngamwongsatit, P., Banada, P.P. Panbangred, W., and Bhurnia, A.K. (2008) WST-1-based cell cytotoxicity assay as a substitute for MTT-based assay for rapid detection of toxigenic Bacillus species using CHO cell line. J. Microbiol. Methods. 73:211-215.

 

50. Thamthiankul, S., Tantichodok, A. and Panbangred, W. (2008)  Spore stage expression of a vegetative insecticaidal gene increases toxicity of Bacillus thuringiensis subsp. aizawai SP41 against Spodoptera exigua. J. Biotechnol.

51. Swanjinda, D., Pala-or, K. and Panbangred, W. (2009)  Simultaneous detection of pediocin gene and species differentiation between Pediococcus acidilactici and P. pentosaceus in a one-step multiplex-overlapping PCR method. Food Biotech. 23:179-189.

52. Igarashi, Y., Shimasaki, R., Miyanaka, S., Oku, N., Onaka, H., Sakurai, H., Saiki, I., Kitani, S., Nihira, T., Wimonsiravude, W. and Panbangred, W. (2010)  Rakicidin D, an inhibitor of turnor cell  invasion from marine-derived Streptomyces sp. J. Antibiot. 63:563-565.

53. Eaunorasetr, J., Nilavongae, A., Tantimavanich, S., Nihira, T., Igarashi, Y. and  Panbangred, W. (2010)  Identification and characterization of soil-isolated Streptomyces SJE177 producing actinomycin. Southeast Asian. J. Trop. Med. Pub. Health 41(5): 1177-1187.

54. Intra, B., Mungsuntisuk, I., Nihira, T., Igarashi, Y., & Panbangred, W. (2011).  Identification of actinomycetes from plant rhizospheric soils with inhibitory activity against Colletotrichom spp., The causative agent of anthracnose disease. BMC. Res. Notes. 4:98-107.

55. Kiatpapan, P. Phonghatsabun, M., Yamashita, M., Marooka, Y. and Panbangred, W. (2011).  Production of 5-aminolevulinic acid by Propionibacterium acidipropionici TISTR442. J. Biosci. Bioeng. 111(4): 425-428.

56. Saenna, P., Gilbreath, T., Onpan, N. and Panbangred, W. (2011).  Actinomycetes community from starch factory wastewater. Res. J. Microbiol. 6(6) : 534-542. 

57. Kaewprapan,K., Wongkongkatep, J., Panbangred,W.,  Phinyocheep, P., Marie, E.,  Durand, A., and Inprakhon, P. (2011).  Lipase-catalyzed synthesis of hydrophobically modified dextrans: Activity and regioselectivity of lipase from Candida rugosa. J. Biosci. Bioeng. 112(2) : 124-129. 

58. Siriwach, R., Kinoshita, H., Kitani, S., Igarashi, Y., Pansuksan, K., Panbangred, W. and Nihira, T. (2011). Xylaropyrone, a new  g-pyrone from the endophytic fungus Xylaria feejeensis MU18. J. Antibiot. 64 : 217-219.

59. Aunpad, R. and Panbangred, W. (2012).  Evidence for two putative holing-like peptides encoding genes of Bacillus pumilus strain WAP34. Cur. Microbiol. 64:343-348. 

60. Buasri, W. and Panbangred, W. (2012). Large crystal toxin formation in chromosomally engineered Bacillus thuringiensis subsp. aizawai due to sE accumulation. Appl. Environ. Microbiology. 78(6):1682-1691.

61. Igarashi, Y., Tanaka, Y., Ikeda, M., Oikawa, T., Kitani, S., Nihira, T., Mongkol. P., Janhom, M. and Panbangred, W. (2012).  Prajinamide, a new modified peptide from a soil-derived Streptomyces.. J. Anitibiot. 65(3) : 157-159.

62. Buasri, W., Impoolsup, A., Boonchird, C., Luengchaichawange, A., Prompiboon, P., Petre, J. and Panbangred, W. (2012). Construction of Bordetella pertussis strains with enhanced production of genetically-inactivated Pertussis Toxin and Pertactin by unmarked allelic exchange. BMC Microbiology 12 : Article number 61.

63. Igarashi, Y., Yu, L., Ikeda, M., Oikawa, T., Kitani, S., Nihira, T., Bayanmunkh, B., and Panbangred, W. (2012). Jomthonic acid , a modified amino acid from a soil-derived Streptomyces. J. Natural Products. 75 (5) : 986-990.

64. Siriwach, R., Kinoshita, H., Kitani, S., Igarashi, Y., Pansuksan, K., Panabngred, W. and Nihira, T. (2012)  Mycoleptione, a new chromosome derivative isolated from the endophytic fungus Mycoleptodiscus sp. MU41. J. Antibiot. 65:627-629.

65. Peng-Ubol, T., Phinyocheep, P., Daniel, P., Panbangred, W., Pilard, J., Thouand, G. and Durand-Thouand, M. (2012). Plasma-treated polyethylene film: A smart material applied for Salmonella Typhimurium detection. Materials Sci. Eng. C32. 2641-2648.

66. Huillet, E., Tempelaars, M.H., André-Leroux, G., Wanapaisan, P., Bridoux, L., Makhzami, S., Panbangred, W., Martin-Verstraete, I., Abee, T. and Lereclus, D. (2012)  PlcRa, a new quorum-sensing regulator from Bacillus cereus, plays a role in oxidative stress responses and cysteine metabolism in stationary phase. PLoS ONE 7(12) : e51047 doi: 10.1371/journal.pone.0051047.

67. Intra, B., Matsumoto, A., Inhashi, Y., Omura, S., Takahashi, Y. and Panbangred, W. (2013)  Actinokineospora bankokensis sp. no., isolated from rhizospheric soil in Thailand. Int. J. Syst. Evol. Microbiol. 63: 2655–2660.

68. Kitani, S., Tomio, A., Srichaisupakit, A., Daduang, R., Intra, B., Panbangred, W., Oku, N., Igarashi, Y. and Nihira, T. (2013)  Sarmentosamide, a novel hexadienamide from Thai soil actinomycetes. Nat. Prod. Res. 27(3) :226-231.

69. Chankhamhaengdecha, S., Hongvijit, S., Srichaisupakit, A., Charnchai, P. and Panbangred, W. (2013)  Endophytic Streptomyces: a novel source of potential acyl homoserine lactone degrading enzyme. BioMed Res. Int. Volume 2013, Article ID782847, 8 pages.

70. Wanapaisan, P., Chumsakul, O. and Panbangred, W. (2013) Enhanced Cry1Da production in Bacillus thuringiensis by driving expression from the sE-dependent BtI promoter. J. Appl. Microbiol. 115 : 859-871.

71. Siriwich, R., Kinoshita, H., Kitani, S., Igarashi, Y., Pansuksan, K., Panbangred, W. and Nihira, T. (2013)  Biopolamides A and B, triene amide isolated from the endophytic fungus Bipolaris sp. MU34. J. Antibiot. (In Press).

 



Last updated : 24/09/2013

customizable counter

View My Stats
Copyright ©2013 Department of Biotechnology, Mahidol University.  All Rights Reserved
Web master :
scctk@mahidol.ac.th