Dr. Chuenchit  Boonchird
 Group : Molecular and Medical Biotechnology
Contact Details :
Room BT111
Department of Biotechnology, Faculty of Science, Mahidol University
Rama VI Road, Ratchathewi, Bangkok 10400, Thailand
Tel :
+66(0)2-201-5304, Mobile: +66(0)89968-5897 Fax : +66(0)2-354-7160
E-mail :
sccbc@mahidol.ac.th
Academic Position :
Mar 2007

Associate Professor, Department of Biotechology, Faculty of Science, Mahidol University

Mar 1994

Assistant Professor, Department of Biotechology, Faculty of Science, Mahidol University

Aug 1986 Assistant Professor, Department of Microbiology, Faculty of Science, Mahidol University
Jan 1981 Lecturer, Department of Microbiology, Faculty of Science, Mahidol University
Education :
Jul 1991

Ph.D. (Molecular Biology) Free University of Brussels, Brussels, Belgium

Jan 1981 M.Sc. (Microbiology) Mahidol University, Bangkok, Thailand
Mar 1977 B.Sc. (Hons) (Medical Technology) Mahidol University, Bangkok, Thailand
Training :

 

Sep 2002           

Training in Yeast Molecular Biology, National Institute of Health, Bethesda, Maryland, USA

 

Oct 1995

Certificate, International Training Program in Biotechnology, Gesellschaft für Biotechnologishe Forchung mbH (GBF), Braunschweig, Germany

 

Sep 1983

Diploma (Microbiology and Biotechnology), International Postgraduate University Course in Microbiology, Osaka University, Osaka, Japan


Awards :

 

Oct 2006           

Outstanding Lecturer Award (Council of the Faculty Senates, Faculty of Science, Mahidol University)


Administration :
  2006-present Chairman, Department of Biotechnology, Faculty of Science, Mahidol University
  2006-present Chairman, Graduate Program Committee, Department of Biotechnology, Faculty of Science, Mahidol University
  2002-2005 Public Sector and International Relations, Faculty of Graduate Studies, Mahidol University
  1998-1999 Associate Dean, Academic Division, Faculty of Science, Mahidol University
  1994-1997 Deputy Chairman, Department of Biotechnology, Faculty of Science, Mahidol University

Expert :
  2010

Mahidol University Representative Committee of NRCT-JSPS Cooperative Project

  2008-present

Deputy Chairman of Thai Society for Biotechnology

  2008-present Education Quality Assurance Surveyor
  2006 Research Proposal Evaluation Committee, TRF
  2006 Risk Assessment on GM Food Additive Committee, BIOTEC
  1994-present Research Proposal and Progress Report Evaluation Committee, BIOTEC
Research Interest :

1. Genetics of thermotolerance in yeast
2. Vaccine development by yeast
3. Evaluation of estrogenic activity in Thai medicinal plant by yeast based assay
4. Screening of bioactive compounds from endophytic fungi

Research Grant:

1.   Bordetella” BioNet-Asia Co.Ltd., 2007-2010, principle investigator.

2.   “Testing of Heterologously Expressed Viral Coat Protein for Use in Production of Specific Pathogen Free (SPF) Shrimp Broodstock and Larvae” National Center for Genetic Engineering and Biotechnology Grant, 2007-2009, co-investigator.

3.  Thailand-Japan Bilateral Scientific Co-operation in the Field of Biotechnology, “Subproject: Genetic and Genomic Approaches to Design Thermotolerant Strain of Yeast for High Level Ethanol Production” National Research Council Thailand, 2006-2009, principle investigator.

Publications :

1.     Cherdshewasart W, Mahapanichkul T and Boonchird C*. Estrogenic and anti-estrogenic activities of the Thai traditional herb, Butea superba Roxb. Biosci Biotech Bioch. 2010;74:2176-82.p>

2.     Moukamnerd C, Kino-oka M, Sugiyama M, Kaneko Y, Boonchird C, Harashima S, Ninimiya K, Shioya S and Katakura Y. Ethanol production from biomass by repetitive solid-state fed-batch fermentation with continuous recovery of ethanol. App Microbiol Biotech 2010;88:87-94.

3.     Tammawong S, Ninomiya S, Kawasaki H, Boonchird C, and Sumpradit T*. Millerozyma phetchabunensis sp. nov., a novel ascomycetous yeast species isolated from Nam Nao forest soil in Thailand, and the transfer of Pichia koratensis to the genus Millerozyma. J Gen Appl Bio 2010;56:37-42.

4.     Boonchird C, Mahapanichkul T, and Cherdshewasart W*. Differential binding with ER-alpha and ER-beta of a phytoestrogen-rich plant Pueraria mirifica. Braz J Med Biol Res 2010;43:195-200.

5.     Auesukaree C*, Damnernsawad A, Kruatrachue M, Pokthitiyook P, Boonchird C, Kaneko Y and Harashima S. Genome-wide identification of genes involved in thermotolerance to various environmental stress in Saccharomyces cerevisiae. J Appl Genet 2009;50:301-10.

6.     Winuthyanon W, Suksen K, Boonhcird C, Chuencharunee A, Ponglikitmongkol M, Suksamrarn A, and Piyachaturawat P*. Estrogenic activity of diarylheptanoids from Curcuma comosa Roxb. requires metabolic activation. J Agric Food Chem 2009;57:840-5.

7.     Koedrith P, Dubois E, Scherens B, Jacobs E, Boonchird C, Messenguy F*. Identification and characterization of a thermotolerant yeast strain isolated from banana leaves. Science Asia 2008;34:147-52.

8.     Thongekkaew J and Boonchird C*. Molecular cloning and functional expression of a novel extracellular lipase gene from the thermotolerant yeast Candida thermophila. FEMS Yeast Research 2007;7:232-43.

9.     Sawamipak S, Sophasan S, Endou H, and Boonchird C*. Functional expression of the rat organic anion transporter1 (OAT1) in Saccharomyces cerevisiae. BBA-Biomembranes 2005;1720:44-51.

10.  Oiu H, HU C, Zhang F, Hwang GJ, Swanson MJ, Boonchird C, and Hinnebusch AG*. Interdependent recruitment of SAGA and Srb mediator by transcriptional activator Gcn4p. Mol Cell Biol 2005;25:3461-74.

11.  Monkongdee P, Boonchird C, Balachandra K, Thawaranantha D, Watanaseree J, and Pantuwatana S*. Cloning and sequence analysis of hepatitis B virus genome of adr subtype isolated in Thailand. J Sci Soc Thailand 1998;24:155-67.

12.  Thawaranantha D, Balachandra K, Boonchird C, Pituksuteepong C, Watanaseree J, and Pantuwatana S*. Nucleotide sequencing of the genes encoding for the middle surface protein from hepatitis B virus genomes subtypes adr and adw in Thailand. J Sci Soc Thailand 1997;23:87-100.

13.  Balachandra K, Supanaranond K, Boonchird C, Buddhirakkul N, Thawaranantha D, Jullaksorn D, Chatanachan P, Watanaseree J, and Pantuwatana S*. Expreesion of hepatitis B virus surface antigen in Cos-7 cells by using recombinant pcDNAI vector. J Sci Soc Thailand 1997;23:41-50.

14.  Thanomsub BW, Boonchird C, and Meevootisom V*. Cloning and nucleotide sequencing of a lipase gene from Bacillus subtilis WRRL-B558. World J Microbiol & Biotech 1996;12:619-23.

15.  Balachandra K, Thawaranantha D, Boonchird C, Techayingpaiboon D, Pitaksutheepong C, Watanaseree J, Supanranon K, and Pantuwatana S*. Cloning and restriction endonuclease analysis of hepatitis B viral genome. J Sci Soc Thailand 1996;22:325-32.

16.  Thawaranantha D, Balachandra K, Watanaseree J, Boonchird C, Warachit P, Bhumisawasdi J, and Pantuwatana S*. Subtyping of Thai hepatitis B virus DNA by polymerase chain reaction. J Sci Soc Thailand 1994;20:115-24.

17.  Boonchird C, Messenguy F*, and Dubois E. Determination of amino acid sequences involved in the processing of the ARG5/ARG6 precursor in Saccharomyces cerevisiae. Eur J Biochem 1991;199:325-35.

18.  Messenguy F*, Dubois E, and Boonchird C. Characterization of the DNA-binding sequences of ARG5,6  proteins to arginine anabolic and catabolic promoters. Mol Cell Biol 1991;11:2852-63.

19.  Boonchird C, Messenguy F*, and Dubois E.  Characterization of the yeast ARG5,6 gene : Determination of  the nucleotide sequence, analysis of the control region and of ARG5,6 transcript. Mol Gen Genet 1991;226:154-66.

20.  Messenguy F., Dubois E, and Boonchird C. Homology between DNA-binding sequences of ARGR regulatory proteins and PRTF and SRF factors. Arch Int Physiol Biochim 1989;98:B36.

21.  Takagi A, Chua EN, Boonchird C, Harashima S, and Oshima Y*. Constant Copy numbers of plasmids in Saccharomyces cerevisiae host with different ploidy. Appl Microbiol Biotechnol. 1985;23:123-9.

22.  Boonchird C, Takagi A, Harashima S, and Oshima Y*. Expression of a cloned gene in isogenic series of Saccharomyces cerevisiae polyploid strains. Annual Reports of International Center of Cooperative Research and Development in Microbiology Engineering, Japan 1983;6:207-19.

23.  Boonchird C and Flegel TW*. In vitro antifungal activity of eugenol and vanillin against Candida albicans and Crytococcus neoformans. Can J Microbial 1982;28:1235-41.

24.  Boonchird C and Flegel TW. Antifungal activity of eugenol and vanillin against Candida albicans and Cryptococcus neoformans. In: Microbial Utilization of Renewable Resources Vol. 2, International Center for Cooperative Research and Development of Microbial Engineering, Faculty of Engineering, Osaka University, Osaka, Japan; 1981. p. 110-120.

Books :

1.     Boonchird C.  General method for handling of bacteria. In: Panbangred W, Udomvaraphan S, editors. Techniques in Molecular Genetics and Genetics Engineering, Thai Society for Biotechnology. Salaya, Nakhon Pathom: ASEAN Institute for Health Development Publisher; 1993. p. 1.1-1.22. (in Thai)

2.     Boonchird C.  Vector for yeast Saccharomyces cerevisiae In: Panbangred W, Udomvaraphan S, editors. Techniques in Molecular Genetics and Genetics Engineering, Thai Society for Biotechnology. Salaya, Nakhon Pathom: ASEAN Institute for Health Development Publisher; 1993. p. 10.52-10.66. (in Thai)

3.     Boonchird  C. Isolation of yeast chromosomal and plasmid DNA In: Panbangred W, Udomvaraphan S, editors. Techniques in Molecular Genetics and Genetics Engineering, Thai Society for Biotechnology. Salaya, Nakhon Pathom: ASEAN Institute for Health Development Publisher; 1993. p. 11.27-11.33. (in Thai)

4.     Boonchird C. Yeast transformation In: Panbangred W, Udomvaraphan S, editors. Techniques in Molecular Genetics and Genetics Engineering, Thai Society for Biotechnology. Salaya, Nakhon Pathom: ASEAN Institute for Health Development Publisher; 1993. p. 14.33-14.39. (in Thai)

5.     Boonchird C. I Isolation of total yeast RNA In: Panbangred W, Udomvaraphan S, editors. Techniques in Molecular Genetics and Genetics Engineering, Thai Society for Biotechnology. Salaya, Nakhon Pathom: ASEAN Institute for Health Development Publisher; 1993. p. 17.6-17.10. (in Thai)

Graduate Student Theses :

1.      Montananuchat, A. Expression of SSD1 gene in Saccharomyces cerevisiae natural isolates with flocculent phenotype. M.Sc. Thesis (Biotechnology); 2008.

2.      Utairangsee, T. Estrogenic activity of the field-grown White Kwao Krua (Pluraria mirifica) evaluated by a yeast estrogen screen (YES), M.Sc. Thesis (Biotechnology); 2008.

3.      Luengchaichaweng C. Microbial community in Satho fermentation evaluated by DGGE. M.Sc. (Biotechnology); 2008.

4.      Koedrith, P. Identification and characterization of genes required for high temperature growth from a natural isolate of Saccharomyces cerevisiae.  Ph.D. (Biotechnology); 2007

5.      Thongekkaew J. Molecular cloning and functional expression of a novel extracellular lipase gene from the thermotolerant yeast Candida thermophila. Ph.D. (Biotechnology); 2006.

6.      Mahasiripanichkul T. Evaluation of estrogenic activity of Kwau Krue plant extract by yeast estrogen screen (YES) method. M.Sc. (Biotechnology), 2006.

7.      Siriluksanamanon A. Expression of cephalosporin C deacetylase gene form Bacillus cereus BT-24 in heterologous hosts. M.Sc. (Biotechnology); 2005.

8.      Nobthai P. Study on the expression of the middle hepatitis B virus surface gene by truncated HXT7 promoter in Saccharomyces cerevisiae. M.Sc. (Biotechnology); 2005.

9.      Sawamipak S. Expression of the rat organic anion transporter 1 (rOAT1) in the yeast Saccharomyces cerevisiae. M.Sc. (Biotechnology); 2004.

10.    Punlungka B. Expression of the rat organic anion transporter gene OAT1 in the methylotrophic yeast Pichia pastoris. M.Sc. (Biotechnology), 2002.

11.    Wongwigkarn J. Expression of the gene encoding hepatitis B virus surface antigen in the yeast Saccharomyces cerevisiae by PHO84 promoter.  M.Sc. (Biotechnology); 2001.

12.    Footrakul S. Study on the expression of hepatitis B surface and its modified genes in Saccharomyces cerevisiae. M.Sc. (Biotechnology); 2000.

13.    Roekring S. Isolation analysis and expression of cephalosporin C deacetylase gene from Bacillus cereus strain BT-24. M.Sc. (Microbiology); 1999.

14.    Jaiporkhuan W. Heterologous expression of the gene encoding hepatitis B virus surface antigen in yeast. M.Sc. (Biotechnology); 1997.

15.    Leangaramgul P. Complementary DNA cloning of Aspergillus niger glucoamylase gene. M.Sc. (Biotechnology); 1996

16.    Monkongdee P. Cloning and sequencing of hepatitis B virus genome adr subtype isolated in Thailand. M.Sc. (Microbiology); 1996.

17.    Thanomsub B. Molecular studies of genes encoding for enzymes capable of hydrolyzing cephalosporin C and lipid. Ph.D. (Microbiology); 1996.


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