Curriculum Vitae

NAME: Worachart Sirawaraporn
DATE OF BIRTH: 1 September 1955
PLACE OF BIRTH: Bangkok , Thailand .
INSTITUTION AFFILIATION: Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand.
EDUCATION:
1974-1978 
B.Sc. in Med. Tech., Faculty of Medicine, Chulalongkorn University.
1979-1980 
M.Sc. in Biochemistry, Faculty of Science, Mahidol University.
1981-1985 
Ph.D. in Biochemistry, Faculty of Science, Mahidol University.
1986-1988 
Post-doctoral fellow, Department of Biochemistry and Biophysics, University of California, San Francisco, U.S.A.

PROFESSIONAL EXPERIENCE:
1982-1988 
Lecturer, Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok Thailand.
1988-1992 
Assistant Professor, Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok Thailand.
1991-2001 
Director, Bioservice Unit, Thailand National Center for Genetic Engineering and Biotechnology.
1992-2001 
Associate Professor, Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok Thailand.
2001-present 
Professor, Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok Thailand.
2002-present 
Director, Center for Bioinformatics and Applied Genomics Research Unit (CBAG), Faculty of Science, Mahidol University, Bangkok Thailand.

PROFESSIONAL AFFILIATION:
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Member, Pathogenesis Steering Committee, TDR/WHO (1995-1999).
  Member, Pathogenesis and Applied Genomics Steering Committee, TDR/WHO (2000-2005).
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Scientific Working Group, Bioinformatics Initiative, TDR/WHO (2001-present)
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Coordinator ( Asia), South-South Initiative, TDR/WHO (2002-present)
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Editorial Board, Trends in Parasitology, Elsevier Science, London, UK (2001-present)
-
Editorial Advisory Board, Molecular Microbiology, Blackwell Science, USA (2001-2004)
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Editorial Board, Science Journal (in Thai) (2003-present)
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Associate Editor, ScienceAsia (2006-present)
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Deputy Chair, Protein Society of Thailand (2006-present)
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Elected member, Asia-Pacific International Molecular Biology Network (Asia-Pacific IMBN) (2001-present)

AWARDS AND HONOURS:
1986-1988 
Rockefeller Foundation Post-doctoral fellowship
1988-1992 
L.W. Frohlich Award Research Fellowship from the New York Academy of Sciences
1991 
Promising Scientist Award from the Foundation for the promotion of Science and Technology, Thailand
1992-1995 
Rockefeller Foundation Career Award
1994-1997 
Research Scholar from Thailand Research Fund
1996-2001 
Career Development Award from National Science and Technology Development Agency
1997 
Outstanding Researcher Award from National Research Council Thailand
1998 
Mahidol University Prize (Research)
2004 
Distinguished Alumni Award, Faculty of Graduate Studies, Mahidol University, Thailand
   
PUBLICATIONS:  
1.
Sirawaraporn, W., Panijpan, B. and Yuthavong, Y. (1982) Plasmodium berghei: Uptake and distribution of chloroquine in infected mouse erythrocytes. Expt. Parasitol. 54, 260-270. 
2.
Sirawaraporn, W. (1984) General techniques for study of parasites: preparation of crude fractions from host-parasite complex. Manual of the international workshop on Genetic Engineering Techniques in Tropical Diseases Research, Faculty of Science, Mahidol University, Bangkok, Thailand. 407-411. 
3.
Sirawaraporn, W. , and Yuthavong, Y. (1984) Kinetic and molecular properties of dihydrofolate reductase from pyrimethamine-sensitive and pyrimethamine-resistant. Plasmodium chabaudi.Mol. Biochem. Parasitol. 10,355-367. 
4.
Yuthavong, Y., Panijpan, B., Ruenwongsa, P., and Sirawaraporn, W. (1985) Biochemical aspects of drug action and resistance in malaria parasites. Southeast Asian J. Trop. Med. Pub. Hlth. 16, 459-472. 
5.
Sirawaraporn, W. and Yuthavong, Y. (1986) Potentiating effect of pyrimethamine and sulfadoxine against dihydrofolate reductase from pyrimethamine-sensitive and pyrimethamine-resistand Plasmodium chabaudi. Antimicrob. Agents Chemother. 29, 899-905. 
6.
Grumont, R., Sirawaraporn, W. and Santi, D.V. (1988) Heterologous expression on the bifunctional thymidylate synthase-dihydrofolate reductase from Leishmania major. Biochemistry 27, 3776-3784. 
7.
Sirawaraporn, W., Sertsrivanich, R., Booth, R.G., Hansch, C., Neal, R.A. and Santi, D.V. (1988) Selective inhibition of Leishmania dihydrofolate reductase and Leishmania growth by 5-benzyl-2, 4-diaminopyrimidines. Mol. Biochem. Parasitol. 31, 79-86. 
8.
Davisson, V.J., Sirawaraporn, W. and Santi, D.V. (1989) Expression of human thymidylate synthase in E. coli. J. Biol. Chem. 264, 9145-9148. 
9.
Sirawaraporn, W., Sirawaraporn, R., Cowman, A.F., Yuthavong, Y. and Santi, D.V. (1990) Heterologous expression of active thymidylate synthase dihydrofolate reductase from Plasmodium falciparum. Biochemistry 29, 10779-10785. 
10.
Sirawaraporn, W., Edman, J.C. and Santi, D.V. (1991) Purification and properties of recombinant Pneumocystis carinii dihydrofolate reductase. Protein Expr. Purif. 2, 313-316. 
11.
Sirawaraporn, W., Sirawaraporn, R., Chanpongsri, A., Jacobs, Jr., W.R. and Santi, D.V. (1991) Purification and characterization of dihydrofolate reductase from wide-type and trimethoprim-resistant Mycobacterium smegmatis. Exp. Parasitol. 72, 184-190. 
12.
Sirawaraporn, W. (1992) Recent advance on thymidylate synthase-dihydrofolate reductase from Plasmodium falciparum . In: Biotechnology and Environmental Science: molecular approaches (Mongkolsuk, S. Ed.) Plenum Press, New York, 35-48. 
13.
Sirawaraporn, W. (1993) Preparation of blotted membrane for protein microsequencing.  In: Protocols in Molecular Parasitlogy (Hyde, J. E. Ed.) Humana Press, Totowa, New Jersey, 431-440. 
14.
Sirawaraporn, W., Cao, M., Edman, J.C. and Santi, D.V. (1993) Cloning expression and characterization of Cryptococcus neoformans dihydrofolate reductase. J. Biol. Chem. 268, 8888-8892. 
15.
Sirawaraporn, W., Prapunwattana, P., Sirawaraporn, R., Yuthavong, Y. and Santi, D.V. (1993) The dihydrofolate reductase domain of Plasmodium falciparum thymidylate synthase-dihydrofolate reductase: gene synthesis, expression, and anti-folate resistant mutants. J. Biol. Chem. 268, 21637-21644. 
16.
Prapunwattana, P., Sirawaraporn, W., Yuthavong, Y. and Santi, D.V. (1996) Chemical synthesis of the Plasmodium falciparum dihydrofolate reductase thymidylate syntase gene. Mol. Biochem. Parasitol. 83, 93-106. 
17.
Gschwend, D.A., Sirawaraporn, W., Santi, D.V. andKuntz, I.W. (1997) Specificity in structure-based drug design: identification of a novel, selective inhibitor of Pneumocystis carinni dihydrofolate reductase. Proteins 29, 59-67. 
18.
Sirawaraporn, W., Sathitkul, T., Sirawaraporn, R., Yuthavong, Y. and Santi, D.V. (1997) Antifolate-resistant mutants of Plasmodium falciparum dihydrofolate reductase. Proc. Natl. Acad. Sci. U.S.A. 94, 1124-1129. 
19.
Sirawaraporn, W., Yongkiettrakul, S., Sirawaraporn, R., Yuthavong, Y. and Santi, D.V. (1997) Plasmodium falciparum: Asparagine mutation at residue 108 of dihydrofolate reductase is an optimal antifolate-resistant single mutant. Expl. Parasitol. 87, 245-252. 
20.
Churdboonchart, V., Moss, R.B., Sirawaraporn, W., Smutharaks, B., Sutthent, R., Jensen, F.C., Vacharak, P., Grimes, J., Theofan, G. and Carlo, D.J. (1998) Efect of HIV-specific immune-based therapy in subjects infected with HIV-1 subtype E in Thailand. AIDA. 12, 1521-1527. 
21.
Limsuwan, A., Churdboonchart, V., Moss, R.B., Sirawaraporn, W., Sutthent, R., Smutharaks, B., Glidden, D., Trauger, R., Theofan, G. and Carlo, D. (1998) Safety and immunogenicity of REMUNE in HIV-infected Thai subjects. Vaccine 16, 142-149. 
22.
Mckie, J.H., Douglas, K.T., Chan, C., Roser, S., Yates, R., Read, M., Hyde, J.E., Dascombe, M.J., Yuthavong, Y. and Sirawaraporn, W. (1998) Rational drug design approach for overcoming drug resistance: application to pyrimethamine resistance in malaria. J. Med. Chem. 41, 1367-1370. 
23.
Sirawaraporn, W. (1998) Dihydrofolate reductase and antifolate resistance in malaria.  Drug Resist. Updat. 1, 397-404. 
24.
Nopponpunth, V., Sirawaraporn, W., Greene, P. J., and Santi, D. V. (1999) Cloning and expression of Mycobacterium tuberculosis and Mycobacterium leprae dihydropteroate synthase in Escherichia coli. J. Bacteriol. 181, 6814-6821. 
25.
Baca, A.M., Sirawaraporn, R., Turley, S., Sirawaraporn, W. and Hol, W.G.J. (2000) Crystal structure of Mycobacterium tuberculosis 6-hydroxymethyl-7-8-dihydropteroate synthase in complex with pterin monophosphate: New insight into the enzymatic mechanism and sulfadrug action. J. Mol. Biol. 302, 1193-1212. 
26.
Li, R., Sirawaraporn, R., Chitnumsub, P., Sirawaraporn, W., Wooden, J., Athappily, F., Turley, S. and Hol, W.G.J. (2000) Three dimensional structure of M. tuberculosis dihydrofolate reductase reveals opportunities for the design of novel tuberculosis drugs. J. Mol. Biol. 295, 307-324. 
27.
Macreadie, I., Ginsburg, H., Sirawaraporn, W. and Tilley, L. (2000) Antimalarial drug development and new targets. Parasitol. Today 16, 438-444. 
28.
Rastelli, G., Sirawaraporn, W., Sompornpisut, P., Vilaivan, T., Kamchomwongpaisan, S., Quarrell, R., Lowe, G., Thebtaranonth, Y. and Yuthavong, Y. (2000) Interaction of pyrimethamine, cycloguanil, WR99210 and their analogues with Plasmodium falciparum dihydrofolate reductase: structural basis of antifolate resistance. Bioorg & Med Chem. 8, 1117-1128. 
29.
Yuthavong, Y., Vilaivan, T., Chareonsethakul, N., Kamchonwongpaisan, S., Sirawaraporn, W., Quarrell, R. and Lowe, G. (2000) Development of a lead inhibitor for the A16V+S108T mutant of dihydrofolate reductase from the cycloguanil-resistant strain (T9/94) of Plasmodium falciparum. J. Med. Chem. 43, 2738-2744. 
30.
Sukeepaisarncharoen, W., Churdboonchart, V., Kulpradist, S., Isarangkura Na Ayudthya, B., Rugpao, S., Chandeying, V., Sirawaraporn, W ., Carlo, D., and Moss, R. B. (2001) Long-term follow-up of HIV-1 infected Thai patients immunized with Remune monotherapy. HIV Clin. Trials. 2(5), 391-398. 
31.
Chusacultanachai, S., Thiensathit, P., Tarnchonmpoo, B., Sirawaraporn, W. and Yuthavong, Y. (2002) Novel antifolate resistant mutations of Plasmodium falciparum dihydrofolate reductase selected in Escherichia coli. Mol. Biochem. Parasitol. 120, 61-72. 
32.
Leartsakulpanich, U., Imwong, M., Ppukrittayakamee, S., White, J.M., Snounou, G., Sirawaraporn, W. and Yuthavong, Y. (2002) Molecular characterization of dihydrofolate reductase in relation to antifolate resistance in Plasmodium vivax. Mol. Biochem. Parasitol. 119, 63-73. 
33.
Sirawaraporn, W., Sirawaraporn, R., Yongkiettrakul, S., Anuwatwora, A., Rastelli, G., Kamchonwongpaisan, S. and Yuthavong, Y. (2002) Mutational analysis of Plasmodium falciparum dihydrofolate reductase: the role of aspartate 54 and phenylalanine 223 on catalytic activity and antifolate binding. Mol. Biochem. Parasitol. 121, 185-193. 
34.
Tarnchompoo, B., Sirichaiwat, C., Phupong, W., Intaraudom, C., Sirawaraporn, W., Kamchonwongpaisan, S., Vanichtanankul, J., Thebtaranonth, Y. and Yuthavong, Y. (2002) Development of 2,4-diaminopyrimidines as antimalarials based on inhibition of th S108N and C59R+S108N mutants of dihydrofolate reductase from pyrimethamine-resistant Plasmodium falciparum. J. Med. Chem. 45, 1244-1252. 
35.
Rastelli, G., Pacchioni, S., Sirawaraporn, W., Sirawaraporn, R., Parenti, M.D. and Ferrari, A.M. (2003) Docking and database screening reveal new classes of Plasmodium falciparum dihydrofolate reductase inhibitors. J. Med. Chem. 46, 2834-2845. 
36.
Sardarian, A., Douglas, K.T., Read, M., Sims, P.F., Hyde, J.E., Chitnumsub, P., Sirawaraporn, R. and Sirawaraporn, W. (2003) Pyrimethamine analogs as strong inhibitors of double and quadruple mutants of dihydrofolate reductase in human malaria parasites. Org. Biomol. Chem. 1, 960-964. 
37.
Sompornpisut, P., Wijitkosoom, A., Parasuk, V. and Sirawaraporn, W. (2003) Molecular dynamics simulation of the human apo-dihydrofolate reductase: an investigation of an unstable enzyme. Molecular Simulation 29, 111-121. 
38.
Vilaivan, T., Saesaengseerung, N., Jarprung, D., Kamchonwongpaisan, S., Sirawaraporn, W. and Yuthavong, Y. (2003) Synthesis of solution-phase combinatorial library of 4,6-diamino-1,2-dihydro-1,3,5-triazine and identification of new leads against A16V+S108T mutant dihydrofolate reductase of Plasmodium falciparum. Bioorg. Med. Chem. 11, 217-224. 
39.
Yuvaniyama, J., Chitnumsub, P., Kamchonwongpaisan, S., Vanichtanankul, J., Sirawaraporn, W., Taylor, P., Walkinshaw, M.D. and Yuthavong, Y. (2003) Insights into antifolate resistance from malarial DHFR-TS structures. Nat. Struct. Biol. 10, 357-365. 
40.
Alifrangis, M., Christensen, I.T., Jorgensen, F.S., Ronn, A.M., Weng, J.E., Chen, M., Bygbjerg, I.C., Sirawaraporn, W., Palarasah, Y. and Koch, C. (2004) Homology building as a means to define antigenic epitopes on dihydrofolate reductase (DHFR) from P. falciparum. Malar. J. 3, 16. 
41.
Berglez, J., Iliades, P., Sirawaraporn, W., Coloe, P. and Macreadie, I. (2004) Analysis in Escherichia coli of Plasmodium falciparum dihydropteroate synthase (DHPS) alleles implicated in resistance to sulfadoxine. Int. J. Parasitol. 34, 95-100. 
42.
Chantratita, W., Sukeepaisarncharoen, W., Chandeying, V., Kulpradist, S., Israngkura Na Ayudhtaya, B., Rugpao, S., Sirawaraporn, W., Boonshuyar, C. and Churdboonchart, V. (2004) Delayed progression to AIDS in volunteers treated with long-term HIV-1 Immunogen (REMUNE) therapy in Thailand. HIV Med. 5, 317-325. 
43.
Kamchonwongpaisan, S., Wuarrell, R., Charoensetakul, N., Ponsinet, R., Vilaivan, T., Vanichtanankul, J., Tarnchompoo, B., Sirawaraporn, W., Lowe, G. and Yuthavong, Y. (2004) Inhibitors of multiple mutants of Plasmodium falciparum dihydrofolate reductase and their antimalarial activities. J. Med. Chem. 47, 673-680. 
44.
Kasekarn, W., Sirawaraporn, R., Chahomchuen, T., Cowman, A.F. and Sirawaraporn, W. (2004) Molecular characterization of bifunctional hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase from Plasmodium falciparum. Mol. Biochem. Parasitol. 137, 43-53. 
45.
Massimine, K.M., McIntosh, M.T., Doan, L.T., Atreya, C.E., Gromer, S., Sirawaraporn, W., Elliott, D.A., Joiner, K.A., Schirmer, R.H. and Anderson, K.S. (2006) Eosin B as a novel antimalarial agent for drug-resistant Plasmodium falciparum. Antimicrob. Agents Chemother. 50 (9), 3132-41. 
46.
Churdboonchart, V., Sakondhavat, C., Kulpradist, S., Isarangkura Na Ayudthya, B., Chandeying, V., Rugpao, S., Boonshuyar, C., Sukeepaisarncharoen, W., Sirawaraporn, W., Carlo, D.J. and Moss, R. (2000) A double-blind, adjuvantcontrolled trial of human immunodeficiency virus type 1 (HIV-1) immunogen (Remune) monotherapy in asymptomatic, HIV-1-infected Thai subjects with CD4-Cell counts of >300. Clin. Diagn. Lab. Immunol. 7, 728-733. 
47.
Sandefur, C.I., Wooden, J.M., Quayea, I.K., Sirawaraporn, W. and Sibley, C.H. (2007) Pyrimethamine-resistant dihydrofolate reductase enzymes of Plasmodium falciparum are not enzymatically compromised in vitro. Mol. Biochem. Parasitol. 154, 1-5. 
48.
Doungchawee, G., Sirawaraporn, W., Icksang-Ko, A., Kongtim, S., Naigowit, P. and Thongboonkerd, V. (2007) Use of immunoblotting as an alternative method for serogrouping Leptospira. J. Med. Microbiol. 56, 587-592. 
49. Rattanachuen W, J?nsoon M, Swedferg G, Sirawaraporn W*. (2009) Probing the roles of non-homologous insertions in the N-terminal domain of Plasmodium falciparum hydroxymethylpterin pyrophosphokinase-dihydropteroate synthase. Mol Biochem Parasitol 2009 Dec;168(2):135-42.
50. Banjara MR, Sirawaraporn W, Petmitr S, Imwong M, Joshi AB,Chavalitshewinkoon-Petmitr P. (2009) Characteristics and risk factors ofPlasmodium falciparum malaria in Eastern and Central Nepal. Kathmandu Univ Med J (KUMJ). 2009 Oct-Dec;7(28):378-82.
51. Banjara MR, Imwong M, Petmitr S, Sirawaraporn W, Joshi AB, Chavalitshewinkoon-Petmitr P. Factors associated with regional bias of pfcrt (plasmodium falciparum chloroquine resistance transporter) haplotypes in Napal. Southeast Asian J Trop Med Public Health. 2011 Jan;42(1):1-8.
   
   

Last modified: 31-January-2012
© 2008-2009 Department of Biochemistry, Faculty of Science, Mahidol University