Curriculum Vitae

NAME: Prapon Wilairat
DATE AND PLACE OF BIRTH: June 30, 1944; Bangkok, Thailand
INSTITUTIONAL AFFILIATION: Department of Biochemistry
Faculty of Science, Mahidol University
Rama 6 Road, Bangkok 10400, Thailand
PRESENT POSITION: Professor
Department of Biochemistry
Faculty of Science, Mahidol University
DEGREES AWARDED: B.Sc. (Honours) (Chemistry) (1966)
Australian National University
Ph.D. (Biochemistry) (1974)
University of Oregon, U.S.A.

AWARDS AND HONORS:
 
  • Australian Government Colombo Plan Undergraduate Scholarship, 1963-6
  • Rockefeller Foundation Pre-doctoral Fellowship, 1967-1973
  • Mahidol University Prize in Research, 1987 (shared)
  • Mahidol University Prize in Teaching, 1993
  • National Research Council of Thailand Outstanding Research Award, 1995 (shared)
  • National Research Council of Thailand First Prize for Invention, 1996 (shared)
  • National Research Council of Thailand Outstanding Researcher, 1996
  • Thailand Research Fund Senior Research Scholar Award, 1996-1999, 2000-2003
  • Thailand Outstanding Scientist, Foundation for the Promotion of Science and Technology, 1997
  • Alumni Fellow, College of Arts and Sciences, University of Oregon, 1998
  • University Councils of Thailand Outstanding Teacher Award in Science and Technology, 2004
PROFESSIONAL SERVICE:
  • Life member, Science Society of Thailand Founding member, Thailand Academy of Science and Technology
  • Chairman of the Department of Biochemistry, Faculty of Science, Mahidol University, 1988-1991, 2002-2004
  • Associate Director, Institute of Molecular Biology and Genetics, Mahidol University, 1999-2002
  • Chairman, Biochemistry Section of the Science Society of Thailand, 1988-1989
  • Senior advisor and Board Member, BIOTEC, National Science and Technology Develoment Agency, Ministry of Science and Technolgy, Thailand
  • Editor, Journal of the Science Society of Thailand, 1990-1991
  • Member, Editorial Board, Science Asia (formerly Journal of the Science Society of Thailand)
  • Member, Editorial Board, Asia Pacific Journal of Molecular Biology and Biotechnology
  • Regional Editor, Journal of Biological Sciences
RESEARCH INTERESTS:
  • Biochemistry of malaria
  • Pathophysiology of thalassemia
  • Familial kidney disease
  • Vitamin E function

PUBLICATIONS:

1. Bradbury, H. and Wilairat P. (1967) Proton magnetic resonance spectroscopy of histidine residues in proteins. Biochem Biophys Res Commun 29, 84-89.

2. Woodward, W.R., Adamson, S.D., McQueen, H.M., Larson, J.W., Estvanik, S.M., Wilairat P. and Herbert, E. (1973) Globin synthesis on reticulocyte membrane-bound ribosomes. J Biol Chem 248, 1556-1561.

3. Woodward, W.R., Wilairat P., Herbert, E. (1974) Preparation of reticulocyte aminoacyl-tRNA and the assay of codon recogntion properties of isoacceptor tRNA's in a reticulocyte cell-free system. Methods Enzymol 30, 740-746.

4. Wilairat P., Woodward, W.R. and Herbert, E. (1975) Precision of initiation of globin synthesis in a rabbit reticulocyte cell free system. J. Sci. Soc. Thailand. 1, 188-204.

5. Santiyanont, R., Yaipimol, C. and Wilairat P. (1977) Accumulation of orthochromatophilic normoblasts in bone marrow of vitamin E-deficient monkey, Macaca fasicularis. J. Nutr. 107, 2026-2030.

6. Wilairat P., Kunaporn, C., Pravatmuang, P., Siriwittayakorn, J. and Tantiyavarong, N. (1977) Colchicine does not inhibit rabbit reticulocyte globin synthesis. J. Sci. Soc. Thailand 3, 90-92.

7. Santiyanont, R. and Wilairat P. (1978) Erythrocyte membrane protein pattern of normal and vitamin E-deficient monkey Macaca fascicularis. IRCS Medical Science 6, 317.

8. Wilairat P., Yuthavong, Y. and Khungvanlert, R. (1978) Effect of membrane modification on cell fusion of hen erythrocytes induced by dimethyl sulfoxide. Life. Sci. 22, 1993-1998.

9. Boonjawat, J., Wilairat P. and Vimokesant, S.L. (1979) Alteration in bone marrow RNA of vitamin E-deficient monkey, Macaca fascicularis. Am. J. Clin. Nutr. 32, 2065-2067.

10. Burapakulsolsri, N., Yuthavong, Y. and Wilairat P. (1979) An examination of complement proteins on membranes of paroxysmal nocturnal haemoglobinuria (PNH) and PNH-like red cells. Brit. J. Haematol. 41, 393-398.

11. Vajanamarhutue, C., Wilairat P. and Komaratat, P. (1979) Effects of Vitamin E deficiency on the activities of lipid-requiring enzymes in rabbit liver and muscle. J. Nutr. 109, 848-855.

12. Yuthavong, Y., Wilairat P., Panijpan, B., Potiwan, C. and Beale, G.H. (1979) Alterations in membrane proteins of mouse erythrocytes infected with different species and strains of malaria parasites. Comp. Biochem. Physiol. 63B, 83-85.

13. Jearnpipatkul, A., Govitrapong, P., Yuthavong, Y., Wilairat P. and Panijpan, B. (1980) Binding of antimalarial drugs to hemozoin from Plasmodium berghei. Experientia. 36, 1063-1064.

14. Liewsaree, P., Yuthavong, Y., Wilairat P. and Komaratat, P. (1980) Protein and lipid composition of sarcoplasmic reticulum from dystrophic muscles of vitamin E-deficient rabits. Nutr. Rep. Int. 22, 853-862.

15. Ucchin, P., Komaratat, P. and Wilairat P. (1980) Effect of vitamin E deficiency on fatty acid uptake of rabbit erythrocyte. In Food and Nutritional Biochemistry (Khor HT, Ong KK, Oo KC, ed). Malaysian Biochemical Society, Kuala Lumpur, Malaysia, 237-247.

16. Rungsitiyakorn, A., Wilairat P. and Panijpan, B. (1981) On the pH dependence of binding of berberine to DNA. J. Pharm. Pharmacol. 33, 125-127.

17. Santiyanont, R. and Wilairat P. (1981) Red cells containing hemoglobin E do not inhibit malaria parasite development in vitro. Am. J. Trop. Med. Hyg. 30, 541-543.

18. Wilairat P. and Chaicharoen, S. (1981) Laboratory practicals on the biosynthesis of protein using intact rabbit reticulocytes. Biochem Ed 9, 10-11.

19. Yuthavong, Y. and Wilairat P. (1981) The biochemistry of malaria. Biochem Ed 9, 122-127.

20. Dejkriengkraikhul, P. and Wilairat P. (1982) A rapid method for concentrating schizont-infected red cells of Plasmodium falciparum. Southeast Asian J. Trop. Med. Public Health 13, 663-665.

21. Dejkriengkraikhul, P., Santiyanont, R. and Wilairat P. (1982) The effect of soluble glycophorin A on invasion of human red cells by the malarial parasite Plasmodium falciparum. Southeast Asian J. Trop. Med. Public Health 13, 662-663.

22. Liewsaree, P., Kalumphaheti, C. and Wilairat P. (1982) Studies on sheep red cell membrane: identification of two high molecular weight intrinsic proteins. J. Sci. Soc. Thailand 8, 225-228.

23. Dejkriengkraikhul, P. and Wilairat P. (1983) Requirement of malarial proteases in the invasion of human red cells by merozoites of Plasmodium falciparum. Z. Parasitenkd 69, 313-317.

24. Patipaporn, K., Komaratat, P. and Wilairat P. (1983) Altered property of sarcoplasmic Ca-ATPase from vitamin E-deficient. Biochem. Int. 6(3), 335-338.

25. Santiyanont, R. and Wilairat P. (1983) Identification and localization of antigens of the malaria parasite, Plasmodium falciparum by immune precipitatiion. Biochem. Int. 7, 671-676.

26. Tungpradapkul, S., Panyim, S., Wilairat P. and Yuthavong, Y. (1983) Analysis of DNA from various species and strains of malaria parasites by restriction endonuclease fingerprinting. Comp. Biochem. Physiol. 74B, 481-485.

27. Chupukcharoen, N., Komaratat, P. and Wilairat P. (1985) Effects of vitamin E deficiency on the distribution of cholesterol in plasma lipoproteins and the activity of cholesterol 7alpha-hydroxylase in rabbit liver. J. Nutr. 115, 468-472.

28. Komaratat, P., Chupukcharoen, N. and Wilairat P. (1985) Effect of vitamin E on cholesterol plasma lipoprotein distribution and metabolism in rabbit. Intern. J. Vit. Nutr. Res. 55, 167-171.

29. Panyim, S., Kertbundit, S., Hiranyavasit, W., Wilairat P., Ponglikitmongkol, M., Bhumiratana, A. and Puntuwatana, S. (1985) Molecular cloning and expression in E. coli of delta-endotoxin gene of Bacillus thuringiensis H-14. Microbial. Utilization. of Renewable. Resources. 4, 214-217.

30. Promkhatkaew, D., Komaratat, P. and Wilairat P. (1985) Ascorbic acid-Fe2+ treatment mimics effect of vitamin E deficiency on sarcoplasmic Ca-ATPase of rabbit muscle. Biochem. Intern. 10, 937-943.

31. Wasi, P., Pootrakul, P., Fucharoen, S., Winichagoon, P., Wilairat P. and Promboon, A. (1985) Thalassemia in Southeast Asia: determination of different degrees of severity of anemia in thalassemia. Annals. NY Acad. Sci. 445, 119-126.

32. Wilairat P., Tirawanchai, N., Intapruk, C., Tungpradapkul, S., Sertsrivanich, R., Panyim, S. and Yuthavong, Y. (1985) Recombinant DNA techniques as potential diagnostic means. Annals dell Istituto Superiore di Sanita 21, 299-306.

33. Wilairat P., Tirawanchai, N., Intapruk, C., Tungpradupkul, S. and Panyim, S. (1985) Strain characterization of the human malaria parasite, Plasmodium falciparum, by the use of a cloned parasite DNA probe. Microbial Utilization of Renewable Resources 4, 210-212.

34. Hamakubo, T., Ueda, M., Fucharoen, S., Wilairat P., Pimainork, Y., Kaunagi, R. and Murachi, T. (1986) Estimation of calpain and calpastatin levels in thalassemic red blood cells and the study of membrane protein. In: Contemporary Themes in Biochemistry (ICSU Short Report, Vol. 6), ed. O.L. Kon et al., Cambridge University Press, Singapore. 624-625.

35. Panyim, S., Wilairat P. and Yuthavong, Y. (1986) Application of Genetic Engineering to Research on Tropical Disease Pathogens with Special Reference to Plasmodia. WHO, Geneva. 554

36. Angsuthanasombat, C., Chungjatupornchai, W., Kertbundit, S., Luxananil, P., Settasatien, C., Wilairat P. and Panyim, S. (1987) Cloning and expression of 130-Kd mosquito-larvicidal Epsilon-endotozin gene of Bacillus thuringiensis var. israelensis in Escherichia coli. Mol. Gen. Genet. 208, 384-389.

37. Lamchiagdhase, P., Wilairat P., Bunyaratavej, A., Fucharoen, S. and Sahaphong, S. (1987) Altered structure of spectrin in the two types of hemoglobin H disease. In: Thalassemia Pathophysiology and Management, Part A (Birth Defects Original Article Series, vol. 23, No. 5A) ed. S. Fucharoen, P.T. Rowley and N.W. Paul, Alan R. Liss, Inc., New York, 163-168.

38. Lamchiagdhase, P., Wilairat P., Sahapong, S., Bunyaratvej, A. and Fucharoen, S. (1987) Defective spectrin dimer self-association in thalassemic red cells. Eur. J. Haematol. 38, 246-250.

39. Macotpet, G., Wilairat P., Fucharoen, S. and Wasi, P. (1987) Differential expression of erythrocyte calpain and calpastatin activities in beta0-thalassemia/HbE disease. In: Thalassemia Pathophysiology and Management, Part A (Birth Defects Original Article Series, vol. 23, No. 5A) ed. S. Fucharoen, P.T. Rowley and N.W. Paul, Alan R. Liss, Inc., New York, 275-261.

40. Petmitr, S., Wilairat P., Fucharoen, S. and Wasi, P. (1987) Failure to detect large deletions in the b-globin gene of Thai subjects with b0-thalassemia/HbE. Asia Pacific Communications in Biochemistry, 1, 23-25.

41. Pravatmuang, P., Fucharoen, S. and Wilairat P. (1987) No evidence for a role of disulfide bonds in the attachment of inclusion bodies to the red cell membrane. In: Thalassemia Pathophysiology and Management, Part A (Birth Defects Original Article Series, vol. 23, No. 5A) ed. Fucharoen, S., Rowley, P.T., Paul, N.W. and Liss, Alan R. Inc., New York, 227-230.

42. Promboon, A., Wilairat P., Fucharoen, S. and Wasi, P. (1987) Determination of variable severity of anemia in thalassemia: erythrocyte proteolytic activity. In: Thalassemia Pathophysiology and Management, Part A (Birth Defects Original Article Series, vol. 23, No. 5A) ed. Fucharoen, S., Rowley, P.T., Paul, N.W. and Liss, Alan R. Inc., New York, 249-256.

43. Wilairat P. and Komaratat, P. (1987) Sarcoplasmic reticulum Ca-ATPase in vitamin E deficiency. In: Clinical and Nutritional Aspects of Vitamin E (Hayashi, O. and Mino, M. Eds.) Elsevier Science Publisher, Amsterdam 85-88.

44. Fucharoen, S., Tirawanchai, N., Wilairat P., Panyim, S. and Thaithong, S. (1988) Differentiation of Plasmodium falciparum clones by means of a repetitive DNA probe. Trans. R. Soc. Trop. Med. Hyg. 82, 209-211.

45. Kamolvarin, N., Wilairat P. (1988) Electroblotting and autoradio immunoanalysis of complement terminal complex. In Nature of Genetic Variations in Man (Chauhan PS, Reddy PP, ed. EMSI/BARC, Bombay. 41-52.

46. Nirdnoy, W., Komaratat, P. and Wilairat P. (1988) Comparison of sarcoplasmic reticulum Ca2+-adenosine triphosphatase from vitamin E-deficient dystrophic rabbit skeletal muscle with iron-ascorbate-treated and untreated enzyme. J. Biochem. 103, 309-312.

47. Boonsaeng, V., Chansiri, K., Vilasineekul, P., Wilairat P. Panyim, S. (1989) Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application. Southeast Asian J. Trop. Med. Public Health 20, 519-522.

48. Petmitr, S., Wilairat P., Kownkon, J., Winichagoon, P., Fucharoen, S. (1989) Molecular basis of b0 -thalassemia/Hb E disease in Thailand. Biochem. Biophys. Res. Commun. 162, 846-851.

49. Settasatian, N.C., Wilairat P. and Komaratat, P. (1989) Effect of vitamin E status on hepatic cholesterol-7-b-hydroxylase. In CRC Handbook of Free Radicals and Antioxidants in Biomedicine, Vol II (Miquel, J., Quiantanilha, A.T., Weber, H. ed). CRC Press Inc, Boca Raton, Florida, 161-165.

50. Wilairat P. (1989) DNA probes for malaria parasite detection: current status and future prospects. In: Immunological and Molecular Basis of Pathogenesis in Parasitic Disease (Ko RC, ed). Hong Kong University Press, Hong Kong 169-174.

51. Phonpanichrasamee, C., Komaratat, P. and Wilairat P. (1990) Hypocholesterolemic effect of vitamin E on cholesterol-fed rabbit. Internat. J. Vit. Nutr. Res. 60, 240-244.

52. Chavalitshewinkoon, P. and Wilairat P. (1991) A simple technique for large scale in vitro culture of Plasmodium falciparum. Southeast Asian J. Trop. Med. Public Health. 22, 544-547.

53. Tirasophon, W., Ponglikitmongkol, M., Wilairat P., Boonsaeng, V. and Panyim, S. (1991) A novel detection of a single Plasmodium falciparum in infected blood. Biochem. Biophys. Res. Commun. 175(1), 179-184.

54. Figgitt, D., Denny, W., Chavalitshewinkoon, P., Wilairat P. and Ralph, R. (1992) In vitro study of anticancer acridines as potential antitrypanosomal and antimalarial agents. Antimicrob. Agents Chemother. 36, 1644-1647.

55. Petchpoo, W., Tan-ariya, P., Boonsaeng, V., Brokelman, C.R., Wilairat P. and Panyim, S. (1992) A specific DNA probe which identifies Babesia bovis in whole blood. Vet. Parasitol. 42, 189-198.

56. Thaithong, S., Chan, S.W., Songsomboon, S., Wilairat P., Seesod, N., Sueblinwong, T., Goman, M., Risdley, R. and Beale, G. (1992) Pyrimethamine resistant mutations in Plasmodium falciparum. Mol. Biochem. Parasitol. 52, 149-158.

57. Wilairat P., Kittikalayawong, A. and Chaicharoen, S. (1992) The thalassemic red cell membrane. Southeast Asian J. Trop. Med. Public Health 23, 74-78.

58. Winichagoon, P., Fucharoen, S., Wilairat P., Chihara, K., Fukumaki, Y. and Wasi, P. (1992) Identification of five rare mutations including a novel frame shift mutation causing beta0 - thalassemia in Thai patients with beta0 - thalassemia/hemoglobin E disease. Biochim. Biophys. Acta. 1139, 280-286.

59. Chavalitshewingkoon, P., Wilairat P., Gamage, S., Denny, W., Figgitt, D. and Ralph, R. (1993) Structure-activity relationships and modes of action of 9-anilinoacridines against chloroquine-resistant Plasmodium falciparum in vitro. Antimicrob. Agents Chemother. 37, 403-406.

60. Kittiwatanapaisan, W., Wilairat P., Kotchabhakdi, N. and Govitrapong, P. (1993) Effect of dietary vitamin E on L-[3H] glutamate binding in rabbit cerebellum. J. Nutr. Sci. Vitaminol. 39, 81-88.

61. Petmitr, S., Fochareon, S., Rajkulchai, P., Bifani, J.P. and Wilairat P. (1993) beta0-thalassemia due to a cytosine deletion in codon 41 associated with hemoglobin E. Southeast Asian J. Trop. Med. Public health. 24, 384-385.

62. Tassanakajon, A., Boonsaeng, V., Wilairat P. and Panyim, S. (1993) Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes. Trans. R. Soc. Trop. Med. Hyg. 87, 273-275.

63. Tirasophon, W., Tassanakajon, A., Boonsaeng, V., Panyim, S. and Wilairat P. (1993) Sensitive detection of Plasmodium falciparum in blood and mosquito by DNA amplification. Parassitologia 35, 117-120.

64. Wilairat P. and Komaratat, P. (1993) Hypocholesterolemic effect of vitamin E. Biochemistry (Life Sci. Adv.) 12, 53-55.

65. Winichagoon, P. Thonglairoam, V., Fuchareon, S., Wilairat P., Fukumaki, Y. and Wasi, P. (1993) Severity differences in beta-thalassemia/haemoglobin E syndromes: implication of genetic factors. Br. J. Haematol. 83, 633-639.

66. Yuthavong, Y. and Wilairat P. (1993) Protection against malaria by thalassemia and haemoglobin variants. Parasitol. Today 9, 241-245.

67. Chavalitshewinkoon, P., Leelaphiwat, S. and Wilairat P. (1994) Partial purification and characterization of DNA topoisomerase II from Plasmodium falciparum. Southeast Asian J. Trop. Med. Public Health 25, 32-36.

68. Gamage, S.A., Tepsiri, N., Wilairat P., Woljcik, S.J., Figgitt, D.P., Ralph, R.K. and Denny, W.A. (1994) Synthesis and in vitro evaluation of 9-anilino-3, 6-diaminoacridines active against a multidrug-resistant strain of the malaria parasite Plasmodium falciparum. J. Med. Chem. 37, 1486-1494.

69. Ponglikitmongkol, M., Duangruitai, N., Boonsaeng, V. Wilairat P. and Panyim, S. (1994) Field evaluation of simplified radioactive and non radioactive DNA probe method for the diagnosis of falciparum malaria. Southeast Asian J. Trop. Med. Public Health 25, 430-435.

70. Tirasophon, W., Rajkulchai, P., Ponglikitmongkol, M., Wilairat P. Boonsaeng, V. and Panyim, S. (1994) A highly sensitive rapid and simple PCR-based method to detech human malaria (Plasmodium falciparum and Plasmodium vivax) in blood samples. Am. J. Trop. Med. Hyg. 51, 308-313.

71. Winichagoon, P., Fucharoen, S., Wilairat P., Chihara, K. and Fukumaki, Y. (1994) Nondeletion type of hereditary persistance of foetal hemoglobin (HPHF): molecular characterization of three unrelated Thai HPHF. Br. J. Haematol. 87, 797-804.

72. Yoosok, C., Bhattarakosol, P., Wilairat P. and Sriurairatna, S. (1994) Encapsidation defectiveness of herpes simplex virus type 2 during replication at acid pH condition. Asian Pacific J. Allergy. Immunol. 12, 59-64.

73. Kalpravidh, R.W., Komolvanich, S., Wilairat P. and Fucharoen, S. (1995) Globin chain turnover in reticulocytes from patients with beta0-thalassemia/Hb E disease. Eur. J. Haematol. 55, 322-326.

74. Petmitr, P, Pongvilairat, G. and Wilairat P. (1995) Development of cultivation technique for pure Plasmodium falciparum gametocytes. Southeast Asian J. Trop. Med. Publ. Hlth. 26, 606-610.

75. Winichagoon, P., Fucharoen, S., Wilairat P. and Fukumaki, Y. (1995) Molecular mechanisms of thalassemia in Southeast Asia. Southeast Asian J. Trop. Med. Publ. Hlth. 26, 235-240.

76. Winichagoon, P., Fucharoen, S., Wilairat P., Chihara, K. and Fukumaki, Y. (1995) Role of alternatively spliced beta E-globin mRNA on clinical severity of beta-thalassemia/hemoglobin E disease. Southeast Asian J. Trop. Med. Publ. Hlth. 26, 241-245.

77. Fucharoen, S., Sae-Ung, N., Rattanasrethong, M., Bunjaratvej, A. and Wilairat P. (1996) Hematological problems in Asian traditional medicine. In: Microcirculatory Approach to Asian Traditional Medicine: strategy for the scientific evalution. (Niimi, H., Xiu, R.J., Sawada, T. and Zheng, C., Eds.) Elsevier Press, Amsterdam. 155-164.

78. Peerapittayamongkol, C., Bernini, L.F. and Wilairat P. (1996) Presence of alphaCS-globin on membrane of red cell containing hemoglobin Constant Spring (CS). J. Sci. Soc. Thailand 22, 117-120.

79. Prasartkaew, S., Zijlstra, N.M., Wilairat P., Overdulve, J.P. and de Vries, E. (1996) Molecular cloning of Plasmodium falciparum gene interrupted by 15 introns encoding a functional pronase 53 kDa subunit as demonstrated by expression in a baculovirus system. Nucleic Acids Res. 24, 3934-3941.

80. Sonthayanon, P., Wilairat P. and Panyim, S. (1996) Molecular cloning, sequencing and amplification of Plasmodium vivax-specific DNA for use in diagnosis by PCR. Asia Pac. J. Mol. Biol. Biotechnol. 4, 118-122.

81. Banjerdpongchai, R., Wilairat P., Fucharoen, S. and Bunyaratvej, A. (1997) Morphological alterations and apoptosis of endothelial cells induced by thalassemic serum n vitro. Southeast Asian J. Trop. Med. Publ. Hlth. 28(3), 149-154.

82. Budimulja, A.S., Syafruddin, Tapchaisri, P., Wilairat P. and Marzuki, S. (1997) The sensitivity of Plasmodial protein synthesis to prokaryotic ribosomal inhibitors. Mol. Biochem. Parasitol. 84, 137-141.

83. Pakdeesuwan, K., Sirinpanyaphinyo, U., Pramoonjago, P., Pattanapanyasat, K., Wilairat P., Kinoshita, T. and Wanachiwanawin, W. (1997) Genotypic and phenotypic implications in paroxysmal nocturnal hemoglobinuria (PNH): a preliminary investigation. Southeast Asian J. Trop. Med. Publ. Hlth. 28(3), 58-63.

84. Pakdeesuwan, K., Wilairat P. and Wanachiwanawin, W. (1997) Paroxysmal nocturnal hemoglobinuria (PNH): from basic research to clinical points of view. Mahidol Univ. J. 4,29-34.

85. Petmitr, P., Pongvilairat, G. and Wilairat P. (1997) Large scale culture technique for pure Plasmodium falciparum gametocytes. Southeast Asian J. Trop. Med. Publ. Hlth. 28, 18-21.

86. Vajrabhaya, L., Sithisarn, P., Wilairat P. and Leelaphiwat, S. (1997) Comparison between sulphorhodamine-B dye staining and 51 Cr-release method in cytoxicity assay of endodontic sealers. J. Endodontics 23, 355-357.

87. Yuthavong, Y. and Wilairat P. (1997) Is the high incidence of malaria in beta-thalassaemic children evidence against the malaria hypothesis? Parasitol.Today 13, 207-208.

88. Fucharoen, S., Chantharaksri, U., Wilairat P., Bunyaratvej, A. and Pootrakul, P. (1998) Oxidative stress in thalassemia: a reappraisal. Trends Biomed Finland Suppl 1, 15-16.

89. Go, M.L., Ngiam, T.L., Tan, A.L.C., Kuaha, K. and Wilairat P. (1998) Structure activity relationships of some indolo [3,2-c] quinolines with antimalarial activity. Eur. J. Pharm. Sci. 6, 19-26.

90. Vattanaviboon, P., Wilairat P. and Yuthavong, Y. (1998) Binding of dihydroartemisinin to hemoglobin H: Role in drug accumulation and host induced antimalarial infectiveness of alpha-thalassemic erythrocytes. Mol. Pharm. 53, 492-496.

91. Kaitatcharachai, C., Vasuvattakul, S., Yenchitsomanus, P., Thuwajit, P., Malasit, P., Chuawatana, D., Maingkum, S., Halperin, L.M., Wilairat P. and Nimmannit, S. (1999) Distal renal tubular acidosis and high urine carbon dioxide tensiion in a patient with Southeast Asian ovalocytosis. Am. J. Kidney Dis. 33, 1147-1152.

92. Thongnoppakhun, W., Wilairat P., Vareesangthip, K. and Yenchitsomanus, P. (1999) Long RT-PCR amplification of the entire coding sequence of the polycystic kidney disease 1 (PKDl) gene. BioTechniques 26, 126-132.

93. Vasuvattakul, S., Yenchitsomanus, P., Vachuanichsanong, P., Thuwajit, P., Kaitwatcharachai, C., Laosombat, V., Malasit, P., Wilairat P. and Nimmannit, S. (1999) Autosomal recessive distal renal tubular acidosis associated with Southeast Asian ovalocytosis. Kidney Int. 56, 1674-1682.

94. Auparakkitanon S, Wilairat P.. Cleavage of DNA induced by 9-anilinoacridine inhibitors of topoisomerase II in the malaria parasite Plasmodium falciparum. Biochem Biophys Res Commun 2000; 269: 406-9.

95. Charoenteeraboon J, Kamchonwongpaisan S, Wilairat P., Vatanaviboon P, Yuthavong Y. Inactivation of artemisinin by thalassemic erythrocytes. Biochem Pharmacol 2000; 59: 1337-44.

96. Chavalitshewinkoon-Petmitr P, Chawprom S, Naesens L, Balzarini J, Wilairat P.. Partial purification and characterization of mitochondrial DNA polymerase from Plasmodium falciparum . Parasitol Intern 2000; 49: 279-88.

97. Chavalitshewinkoon-Petmitr P, Pongvilairat G, Auparakkitanon S, Wilairat P.. Gametocytocidal activity of pyronaridine and DNA topoisomerase II inhibitors against mutidrug-resistant Plasmodium falciparum in vitro. Parasitol Intern 2000; 48: 275-80.

98. Pakdeesuwan K, Wanachiwanawin W, Siripanyaphinyo U, Pattanapanyasat K, Wilairat P., Issaragrisil S. Immunophenotypic discrepancies between granulocytic and erythroid lineages in peripheral blood of patients with paroxysmal nocturnal harmoglobinuria. Eur J Haematol 2000; 65: 8-16.

99. Thongnoppakhun W, Rungroj N, Wilairat P., Vareesangthip K, Sirinavin C, Yenchitsomanus Pt. A novel spice-acceptor site mutation (IVS13-2A>T) of polycystic kidney disease 1 (PKD1) gene resulting in an RNA processing defect with a 74-nucleotide deletion in exon 14 of the mRNA transcript. Hum Mutat 2000; 15: 115.

100. Watanapokasin Y, Winichagoon P, Fucharoen S, Wilairat P.. Relative quantitation of mRNA in ?-thalassemia/Hb E using real-time PCR. Hemoglobin 2000; 2: 105-16.

101. Wilairat P.. Renal tubular acidosis, anaion exchanger 1 (AE1) mutations, Southeast Asian ovalocytosis: a malaria connection? FAOPS Newsletter 2000; 9: 3-8.

102. Chavalitshewinkoon-Petmitr P, Pongvilairat G, Ralph RK, Denny WA, Wilairat P.. Inhibitory effects of 9-anilinoacridines on Plasmodium falciparum gametocytes. Trop Med Int Health 2001; 6: 42-5.

103. Chavalitshewinkoon-Petmitr P, Worasing R, Wilairat P.. Partial purification of mitochondrial DNA topoisomerase II from Plasmodium falciparum and its sensitivity to inhibitors. Southeast Asian J Trop Med Publ Hlth 2001; 32: 733-8.

104. Chaiyen P, Suadee C, Wilairat P.. A novel two-protein component flavoprotein hydroxylase: p-hydroxyphenylacetate from Acinetobacter baumannii. Eur J Biochem 2001; 268: 5550-61.

105. Lui M, Wilairat P., Go ML. Antimalarial alkoxylated and hydroxylated chalcones: structure-activity relationship analysis. J Med Chem 2001; 44: 4443-52.

106. Rungroj N, Thongnoppakhun W, Vareesangthip K, Sirinavin C, Wilairat P., Yenchitsomanus P. Molecular defect of PKD1 gene resulting in abnormal RNA processing in a Thai family. J Med Assoc Thailand 2001; 84: 1308-16.

107. Siripurkpong P, Yuvaniyama J, Wilairat P., Goldberg DF. Active site contribution to specificity of the aspartic proteases plasmepsin I and II. J Biol Chem 2002; 277: 41009-13.

108. Sriwilaijareon N, Petmitr S, Mutirangkura A, Ponglikitmongkol M, Wilairat P.. Stage specificity of Plasmodium falciparum telomerase and its inhibition by berberine. Parasitol Intern 2002; 51: 99-103.

109. Tohtong R, Metheenukul P, Wilairat P.. Status of red cell membrane protein phosphorylation in thalassemia. ScienceAsia 2002; 28: 313-7.

110. Turpaiboon C, Svasti S, Sawangareetakul P, Winichagoon P, Srisomsap C, Siritanaratkul N, Fucharoen S, Wilairat P., Svasti J. Hb Siam [?15(A13)Gly?Arg (?1) (GGT?CGT)] is a typical ? chain hemoglobinopathy without an ?-thalassemic effect. Hemoglobin 2002; 26: 77-81.

111. Vattanaviboon P, Siritanaratkul N, Ketpirune J, Wilairat P., Yuthavong Y. Membrane heme as a host factor in reducing effectiveness of dihydroartemisinin. Biochem Pharmacol 2002; 64: 91-8.

112. Yenchitsomanus P, Vasuvattakul S, Kirdpon S, Wasanawatana S, Susaengrat W, Sreethiphayawan S, Chuawatana D, Mingkum S, Sawasdee N, Thuwajit P, Wilairat P., Malasit P, Nimmannit S. Autosomal recessive distal renal tubular acidosis caused by G701D mutation of anion exchanger 1 gene. Am J Kid Dis 2002; 40: 21-9.

113. Wu X, Wilairat P., Go M-L. Antimalarial activity of ferrocenyl chalcones. Bioorg Med Chem Lett 2002; 12: 2299-302.

114. Auparakkitanon S, Noonpakdee W, Ralph RK, Denny WA, Wilairat P.. Antimalarial 9-anilinoacridine compounds directed at hematin. Antimicrob Agents Chemother 2003; 47: 3708-12.

115. Chavalitshewinkoon-Petmitr P, Ramdja M, Kajorndechakiat S, Ralph RK, Denny WA, Wilairat P.. In vitro sensitivity of Trichomonas vaginalis to AT-specific minor groove binding drugs. J Antimicrob Chemother 2003; 52: 287-9.

116. Liu M, Wilairat P., Croft SL, Tan AL, Go ML. Structure-activity relationships of antileishmanial and antimalarial chalcones. Bioorg Med Chem 2003; 11: 2729-38.

117. Noonpakdee W, Phothikasikorn J, Nimitsantiwong W, Wilairat P.. Inhibition of Plasmodium falciparum proliferation in vitro by antisense oligodeoxynucleotides against malarial topoisomerase II. Biochem Biophys Res Commun 2003; 302: 659-64.

118. Suthiphongchai T, Promyart P, Virochrut S, Tohtong R, Wilairat P.. Involvement of ERK1/2 in invasiveness and metastatic development of rat prostatic adenocarcinoma. Oncology Res 2003; 13: 253-9.

119. Go M-L, Liu M, Wilairat P., Rosenthal PJ, Saliba KJ, Kirk K. Anti-plasmodial chalcones inhibit sorbitol-induced hemolysis of Plasmodium falciparum-infected erythrocytes. Antimicrob Agents Chemother 2004; 48: 3241-5.

120. Smilkstein M, Sriwilaijaroen N, Kelly JX, Wilairat P., Riscoe M. Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening. Antimicrob Agents Chemother 2004; 48: 1803-6.

121. Sritippayawan S, Sumboonnanonda A, Yasuvattakul S, Keskanokwong T, Sawasdee N, Paemanee A, Thuwajit P, Wilairat P., Nimmannit S, Malasit P, Yenchitsomanus P. Novel compound heterozygous SLC4A1 mutations in Thai patients with autosomal distal renal tubular acidosis. Am J Kid Dis 2004; 44: 64-70.

122. Sriwilaijaroen N, Kelly JX, Riscoe M, Wilairat P.. CyQUANT cell proliferation assay as a fluorescence-based method for in vitro screening of antimalarial activity. Southeast Asian J Trop Med Pub Hlth 2004; 35: 840-4.

123. Turbpaiboon C, Siritantikorn A, Thongnoppakhun W, Bunditworapoom D, Limwongse C, Yenchitsomanus P, Siritanaratkul N, Wilairat P.. Hemoglobin Pakse: presence on red blood cell membrane and detection by polymerase chain reaction-single strand conformational polymorphism. Int J Hematol 2004; 80: 136-139.

124. Uthaisang W, Reutrakul V, Krachangchaeng C, Wilairat P., Fadeel B. VR-3848, a novel peptide derived from Euphobiaceae, induces mitochondria-dependent apoptosis in human leukemia cells. Cancer Lett 2004; 208: 171-8.

125. Banjerdpongchai R, Wilairat P.. Effects of water-soluble antioxidants and MAPKK/MEK inhibitor on curcumin-induced apoptosis in HL-60 human leukemic cells. Asian Pac J Cancer Prev 2005; 6: 282-5.

126. Charoenteeraboon J, Nithipatikom K, Campbell WB, Piyachaturawat P, Wilairat P., Rongnoparut P. Induction of human cholesterol 7alpha-hydroxylase in HepG2 cells by 2,4,6-trihydroxyacetophenone. Eur J Pharmacol 2005; 515: 43-6.

127. Dascombe MJ, Drew MG, Morris H, Wilairat P., Auparakkitanon S, Moule WA, Alizadeh-Shekalgourabi S, Evans PG, Lloyd M, Dyas AM, Carr P, Ismail FM. Mapping antimalarial pharmacophores as a useful tool for the rapid discovery of drugs effective in vivo: design, construction, characterization, and pharmacology of metaquine. J Med Chem 2005; 48: 5423-36.

128. Japrung D, Chusacultanachai S, Yuvaniyama J, Wilairat P., Yuthavong Y. A simple dual selection for functionally active mutants of Plasmodium falciparum dihydrofolate reductase with improved solubility. Protein Eng Des Sel 2005; 18: 457-64.

129. Akanitapichat P, Wangmaneerat A, Wilairat P., Bastow KF. Anti-herpes virus activity of Dunbaria bella Prain. J Ethnopharmacol 2006; 105: 64-8.

130. Auparakitanon S, Wilairat P. Antimalarial activity of concanamycin A alone and in combination with pyronaridine. Southeast Asian J Trop Med Public Hlth 2006; 37: 619-21.

131. Auparakkitanon S, Chapoomram S, Kuaha K, Chirachariyavej T, Wilairat P. Targeting of hematin by the antimalarial pyronaridine. Antimicrob Agents Chemother 2006; 50: 2197200.

132. Butthep P, Wanram S, Fucharoen S, Vattanaviboon P, Pattanapanyasat K, Wilairat P. Cytoadherence between endothelial cells and P. falciparum infected and noninfected normal and thalassemic red blood cells. Cytometry B Clin Cytom 2006; 70: 432-42.

133. Kitjaroentham A, Suthiphongchai T, Wilairat P.. Effect of metalloprotease inhibitors on invasion of red blood cell by Plasmodium falciparum. Acta Trop 2006; 97: 5-9.

134. Mahabusarakam W, Kuaha K, Wilairat P. , Taylor WC. Prenylated xanthones as potential antiplasmodial substances. Planta Med 2006; 72: 912-6.

135. Sriwilaijaroen N, Liu M, Go M-L, Wilairat P.. Plasmepsin II inhibitory activity of alkoxylated and hydroxylated chalcones. Southeast Asian J Trop Med Public Hlth 2006; 37: 607-12.

136. Turbpaiboon C, Limjindaporn T, Wongwiwat W, U-Pratya Y, Siritanaratkul N, Yenchitsomanus P-T, Jitrapakdee S, Wilairat P.. Impaired interaction of alpha-haemoglobin stabilising protein with alpha-globin termination mutant in a yeast two-hybrid system. Br J Haematol 2006; 132: 370-3.

137. Wu X, Tiekink ER, Kostetski I, Kocherginsky N, Tan AL, Khoo SB, Wilairat P., Go ML. Antiplasmodial activity of ferrocenyl chalcones: investigations into the role of ferrocene. Eur J Pharm 2006; 27: 175-87.

138. Auparakkitanon S, Poonchareon K, Sopitthummakhum K, Wilairat P.. Interactions between antiplasmodial 3,6-diamino-1-dimethyl-9-anilinoacridine and hematin and concanamycin A. Southeast Asian J Trop Med Public Health 2007; 38: 979-82.

139. Chaisue C, Kitcharoen S, Wilairat P., Jetsrisuparb A, Fucharoen G, Fucharoen S. ?/?-Globin mRNA ratio determination by multiplex quantitative real-time reverse transcription-polymerase chain reaction as an indicator of globin gene function. Clin Biochem 2007; 40: 1373-7.

140. Ponmee N, Chuchue T, Wilairat P., Yuthavong Y, Kamchonwongpaisan S. Artemisinin effectiveness in erythrocytes is reduced by heme and heme-containing proteins Biochem Pharmacol 2007; 74: 153-60.

141. Siritantikorn A, Johansson K, Hlen K, Rinaldi R, Suthiphongchai T, Wilairat P., Morgenstern R. Protection of cells from oxidative stress by microsomal glutathione transferase 1. Biochem Biophys Res Commun 2007; 355: 592-6.

142. Wilairat P.. Local problem, global implication. ScienceAsia 2007; 33 Suppl 1: 81-3.

143. Ganesan K, Ponmee N, Jiang L, Fowble JW, White J, Kamchonwongpaisan S, Yuthavong Y, Wilairat P., Rathod PK. A genetically hard-wired metabolic transcriptome in Plasmodium falciparum fails to mount protective responses to lethal antifolates. PLoS Pathog 2008; 4: e1000214.

144. Sarakul O, Vattanaviboon P, Wilairat P., Fucharoen S, Abe Y, Muta K. Inhibition of alpha-globin gene expression by RNAi. Biochem Biophys Res Commun 2008; 369: 935-8.

145. Sriwilaijaroen N, Kondo S, Yagi H, Wilairat P., Hiramatsu H, Ito M, Ito Y, Kato K, Suzuki Y. Analysis of N-glycans in embryonated chicken egg chorioallantoic and amniotec cells responsible for binding and adaptation of human and avian influenza viruses. Glycoconj J 2009; 26: 433-43.

146. Sriwilaijaroen N, Wilairat P., Hiramatsu H, Takahashi T, Suzuki T, Ito M, Ito Y, Tashiro M, Suzuki Y. Mechanisms of the action of povidone-iodine against human and avian influenza a viruses: its effects on hemagglutination and sialidase activities. Virol J 2009; 6: 124.

147. Thongnoppakhun W, Jiemsup S, Yongkiettrakul S, Kanjanakorn C, Limwongse C, Wilairat P., Vanasant A, Rungroj N, Yenchitsomanus PT. Simple, efficient and cost-effective multiplex genotyping with matrix assisted laser desdorption/ionization time-of-flight mass spectrometry of hemoglobin beta gene mutations. J Mol Diagn 2009; 11: 334-46.

148. Turbpaiboon C, Wilairat P. Alpha-hemoglobin stabilizing protein: molecular function and clinical correlation. Front Biosci. 2010;15:1-11.

149. Sriwilaijaroen, N., Kondo, S., Nanthasri, P., Auparakkitanon, S., Suzuki,Y. and Wilairat, P. Antiplasmodial effect of Brucea javanica (L.)Merr. and Eurycoma longifolia jack extracts and their combination withchloroquine and quinine on Plasmodium falciparum in culture. Trop. Med.Health. 2010; 38 (2):61-68.

150. Limenta LM, Jirasomprasert T, Jittangprasert P, Wilairat P, Yamanont P, Chantharaksri U, Fucharoen S, Morales NP. Pharmacokinetics of deferiprone in patients with beta-thalassaemia: impact of splenectomy and iron status. Clin Pharmacokinet. 2011 Jan 1;50(1):41-50.

   

Last modified: 5-April-2011
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