Curriculum Vitae

NAME: Jirundon Yuvaniyama
DATE OF BIRTH: 14 December 1968
PLACE OF BIRTH: Bangkok , Thailand
B.Sc. (Chemistry) First-Class Honored, Mahidol University, Bangkok, Thailand.
Ph.D. (Biological Chemistry), The University of Michigan, Michigan, USA.
Isolation and differentiation of mitochondrial and genomic DNA of various strains of Bombyx mori (silkworm) by means of restriction fragment-length polymorphism (RFLP) and DNA fingerprinting.
Mentor : Professor Prapon Wilairat, Department of Biochemistry, Mahidol University, Bangkok, Thailand.
Mutagenesis of Escherichia coli thioredoxin reductase: expression, purification, and characterization of the mutant.
Mentor : Professor Charles H. Williams, Jr., Department of Biological Chemistry, The University of Michigan, Michigan, USA.
Purification of Trichosporon cutaneum phenol hydroxylase and pre-steady state kinetic study of its oxidative half-reaction using fluorescence and UV/visible stopped-flow spectrophotometry.
Mentor : Professor Vincent Massey, Department of Biological Chemistry, The University of Michigan, Michigan, USA.
Crystallization and X-ray crystal structure determination of human VH1-related dual specificity phosphatase (VHR) and its peptide-complexes using the techniques of Multiple Isomorphous Replacement (MIR) and Molecular Replacement (MR).
Mentor : Associate Professor Mark A. Saper, Biophysics Research Division, The University of Michigan, Michigan, USA.
Training in Macromolecular Crystallography, Cold Spring Harbor Laboratory, New York, USA.
1994, 1996 
X-ray diffraction data collection at the Cornell High-energy Synchrotron Source (CHESS), Cornell University, USA.
General Employee Radiological Training, Brookhaven National Laboratory, New York, USA.
X-ray diffraction data collection at the National Synchrotron Light Source (NSLS), Brookhaven National Laboratory, New York, USA.
X-ray diffraction data collection at the European Synchrotron Radiation Facility (ESRF), Grenoble, France.
Protein engineering, crystallization, and X-ray structure determination of Plasmodium falciparum plasmepsins: potential targets for antimalarial drug discovery, F. Hoffmann-La Roche, Basel, Switzerland.

Scholarship under the Development and Promotion of Science and Technology Talent Project (DPST), Thailand.
Prof. Thap Nilanithi's Outstanding Graduate Award.
Predoctoral Fellowships, Horace H. Rackham Graduate School, The University of Michigan, Michigan, USA.
The Dominic D. Dziewiatkowski Outstanding Ph.D. Dissertation Award, The University of Michigan, Michigan, USA.
WHO/TDR Training Grant to work on crystallization and X-ray structure determination of potential targets for malarial drug discovery as a visiting scientist at F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Young Scientist Award, Foundation for the Promotion of Science and Technology under the Patronage of H. M. the King, Thailand.

  • Protein X–ray Crystallography
  • Structure-based Drug Design
  • Protein Engineering
Denu, J.M., Zhou, G., Wu, L., Zhao, R., Yuvaniyama, J., Saper, M.A. and Dixon, J.E. (1995) The purification and characterization of human dual specific protein tyrosine phosphatase. J. Biol. Chem. 270, 3796-3803.
Yuvaniyama, J., Denu, J.M., Dixon, J.E. and Saper, M.A. (1996) Crystal structure of the dual specificity protein phosphatase VHR. Science Magazine 272, 1328-1331.
Siripurkpong, P., Yuvaniyama, J., Wilairat, P. and Goldberg, E.D. (2002) Active site contribution to specificity of the aspartic proteases Plasmepsins I and II. J. Biol. Chem. 277, 41009-41013.
Wattanarangsan, J., Chusacultanachai, S., Yuvaniyama, J., Kamchonwongpaisan, S. and Yuthavong, Y. (2003) Effect of N-terminal truncation of Plasmodium falciparum dihydrofolate reductase on dihydrofolate reductase and thymidylate synthase activity. Mol. Biochem. Parasitol. 126, 97-102.
Yuvaniyama, J., Chitnumsub, P., Kamchonwongpaisan, S., Vanichtanankul, J., Sirawaraporn, W., Taylor, P., Walkinshaw, M.D. and Yuthavong, Y. (2003) Insights into antifolate resistance from malarial DHFR-TS structures. Nat. Struct. Biol. 10, 357-365.
Chitnumsub, P., Yuvaniyama, j., Vanichtanankul, J., Kamchonwongpaisan, S., Walkinshaw, M.D. and Yuthavong, Y. (2004) Characterization crystallization and preliminary X-ray analysis of bifunctional dihydrofolate reductase-thymidylate synthase form Plasmodium falciparum. Acta Cryst. 60, 780-783.
Japrung, D., Chusacultanachai, S., Yuvaniyama, J., Wilairat, P. and Yuthavong, Y. (2005) A simple dual selection for functionally active mutants of Plasmodium falciparum dihydrofolate reductase with improved solubility. Protein. Eng. Des. Sel. 18, 457-464.
Kamchonwongpaisan, S., Vanichtanankul, J., Tarnchompoo, B., Yuvaniyama, J., Taweechai, S., and Yuthavong, Y. (2005) Stoichiometric Selection of Tight-Binding Inhibitors of Wild-Type and Mutant Forms of Malarial (Plasmodium falciparum) Dihydrofolate Reductase. Anal. Chem. 77(5), 1222–1227.
Oonanant, W., Sucharitkul, J., Yuvaniyama, J. and Chaiyen, P. (2005) Crystallization and preliminary X-ray crystallographic analysis of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase from Pseudomonas sp. MA-1.  Acta Cryst. 312-314.
Songssiriritthigul, C., Robinson, R.C., Yuvaniyama, J. and Suginta, W. (2005) Expression, purification, crystallization and preliminary crystallographic analysis of chitinase A from Bibrio carchariae. Acta Crystallogr F61(10): 895-898.
Yuthavong, Y., Yuvaniyama, J., Chitnumsub, P., Vanichtanankul, J., Chusacultanachai, S., Tarnchompoo, B., Vilaivan, T. and Kamchonwongpaisan, S. (2005) Malarial (Plasmodium falciparum) dihydrofolate reductase-thymidylate synthase: structureal basis for antifolate resistance and development of effective inhibitors. Parasitology 130, 249-259.
Yuvaniyama, J. (2005) A small step of progress in the ongoing battle against malaria. Siriraj Med. J. 57(10), 457-458.
Chuenchor, W., Pengthaisong, S., Robinson, R.C., Yuvaniyama, J., Oonanant, W., Bevan, D.R., Esen, A., Chen, C., Opassiri, R., Svasti, J. and Cairns, J.R.K. (2008) Structural Insights into Rice BGlu1 beta-Glucosidase Oligosaccharide Hydrolysis and Transglycosylation. J. Mol. Biol. 377, 1200–1215. 
Chuenchor, W., Pengthaisong, S., Yuvaniyama, J., Opassiri, R., Svasti, J. and Ketudat, C.J.R.  (2006) Purification, crystallization and preliminary X-ray analysis of rice BGlu1 Beta-glucosidase with and without 2-deoxy-2-fluoro-Beta-D-glucoside. Acta Cryst. F62, 798–801.
Wongsantichon, J., Yuvaniyama, J. and Ketterman, A.J. (2006) Crystallization and preliminary X-ray crystallographic analysis of a highly stable mutant V107A of glutathione transferase from Anopheles dirus in complex with glutathione. Acta Cryst. 62(Part 3), 310-312.
16. Chuenchor W, Pengthaisong S, Robinson RC, Yuvaniyama J. , Oonanant W, Bevan DR, Esen A, Chen CJ, Opassiri R, Svasti J, Cairns JRK. (2008) Structural insights into rice BGlu1 ?-glucosidase oligosaccharide hydrolysis and transglycosylation. J Mol Biol 377(4):1200-1215.
17. Siritapetawee J, Thammasirirak S, Robinson RC, Yuvaniyama J. (2009) The 1.9 ? X-ray structure of egg-white lysozyme from Taiwanese soft-shelled turtle(Trionyx sinensis wiegmann) exhibits structural differences from the standard chicken-type lysozyme. J Biochem 145(2):193-8.
18. Chuenchor W, Pengthaisong S, Robinson RC, Yuvaniyama J, Svasti J, Cairns JR. The structural basis of oligosaccharide binding by rice BGlu1 Beta-glucosidase. J Struct Biol. 2011 Jan; 173(1):169-79.
19. Budriang C, Rongnoparut P, Yuvaniyama J. An Expression of an Insect Membrane-bound Cytochrome P450 CYP6AA3 in the Escherichia coli in Relation to Insecticide Resistance in a Malarial Vector. Pak J Biol Sci. 2011 Apr 15;14(8):466-75.
20. Vanichtanankul J, Taweechai S, Yuvaniyama J, Vilaivan T, Chitnumsub P, Kamchonwongpaisan S, Yuthavong Y. Trypanosomal dihydrofolate reductase reveals natural antifolate resistance. ACS Chem Biol. 2011 Sep 16;6(9):905-11.
21. Rojviriya C, Pratumrat T, Saper MA, Yuvaniyama J. Improved X-ray diffraction from Bacillus megaterium penicillin G acylase crystals through long cryosoaking dehydration. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1570-4. Epub 2011 Nov 26.

Last modified: 5-April-2011
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