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SC-STAR

NAME:                     Sitthichai Iamsaard (สิทธิชัย  เอี่ยมสะอาด [โก๋])

STUDENT ID:             SCAN/D 4637669

CONTACT:                         E-mail: iamsaard_sitt@yahoo.com

                                             Cell phone: 085-2896363

INSTITUTIONS ATTENDED:

1997 - Dip. in Animal Science  (the highest GPA in the program), Faculty of Animal

Science, Rajamangala University of Technology Suvarnabhumi

Phranakhonsiayutthaya Huntra Campus, Phranakhonsiayutthaya.

1999 - B.Sc. in Technology in Animal Production, Institute of Science and

           Technology for Research and Development, Mahidol University, Bangkok.

2003 - M.Sc. in Anatomy, Faculty of Medicine, Khon Kaen University, Khon Kean.

Present - Ph.D. student in Anatomy and Structural Biology, Faculty of Science,   

               Mahidol University, Bangkok.

Ph.D. RESEARCH GRANTS:

-       University Developing Commissions (UDC) Grant (2003-2006), Ministry of University Affairs, Thailand.

-       Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) grant (2005) to Professor Dr. Boonsirm Withyachumnarnkul  

-       Canadian Institutes of Health Research (CIHR) Grant (2007- May 2009) to Professor Dr. Nongnuj Tanphaichitr, Ottawa Health Research Institute, Ottawa, ON, Canada.

M.Sc. RESEARCH SCHOLARSHIPS:

-          Research Assistant Scholarship (Department of Anatomy, Faculty of Medicine, Khon Kaen University, Thailand) 

-          Graduate Student Scholarship (Graduate School, Khon Kaen University, Thailand)  

-          Research Project scholarship to Associate Professor Dr. Malivalaya Namking  (Faculty of Medicine, Khon Kaen University, Thailand)

AWARDS:

-          The best research poster presentation award (Ph.D. Category), 26th Annual Ottawa Reproductive Biology Workshop, May 14, 2008, Norman Patterson Amphitheatre, the Ottawa Hospital Civic Campus, Ottawa, ON, Canada.

-          Annual Mahidol’s student honors and awards event for 2008 recognizes  student excellence, Faculty of Science, Mahidol University 

Field of interest:

Molecular processes of fertilization (sperm maturation and capacitation, acrosome reaction, sperm-egg interaction, and egg activation), enzymes involved in the fertilization, proprotein convertases, proteomic analyses, and the early development of animals.

Skills:

-          Avian ,cattle, and rodent artificial inseminations

-          Gamete preparations

-          Rodent in vitro fertilization (IVF)

-          In vitro sperm capacitation and acrosome reaction

-          Sperm-egg interactions (cumulus cell-oocyte complex [COC] dispersion, sperm-ZP binding, and sperm-egg plasma membrane binding and fusion)

-          Light and electron microscopy

-          Proteolytic assays

-          Fluorescent zymography in-gel assay

Publications:

1.      Iamsaard, S., Vanichviriyakit, R., Basak, A., Tanphaichitr, N., and Withyachumnarnkul, B. Enzymatic activities in oviductal fluid of black tiger shrimp, Penaeus monodon. (Manuscript in preparation).

2.      Iamsaard, S., Vanichviriyakit, R., Saewu, A., Withyachumnarnkul, B., Basak, A., and Tanphaichitr, N. (2009) Enzymatic activity of proprotein convertase 4 (PC4) is important for mammalian fertilization. (Manuscript is being submitted to PLoS Biology).

3.      Kruevaisayawan, H., Vanichviriyakit, R., Weerachatyanukul, W.,    Iamsaard, S., Withyachumnarnkul, B., Basak, A., Tanphaichitr, N., and Sobhon, P. (2008). Induction of the acrosome reaction in black tiger shrimp (Penaeus monodon) requires sperm trypsin-like enzyme activity. Biol. Reprod. 79, 134-141.

4.      Wu, A.*, Anupriwan, A.*, Iamsaard, S.*, Chakrabandhu, K., Santos, D.C., Rupar, T., Tsang, B.K., Carmona, E., and Tanphaichitr, N. (2007). Sperm surface arylsulfatase A can disperse the cumulus matrix of cumulus oocyte complexes. J. Cell Physiol. 213, 201-211. (* contributing equally to this work)

5.      Kitiyanant Y, Panyarachun B, Kumjantaug T, Seedam C, Tinyoo P, Iamsaard S, Siriaroonrat B. and Pavasuthipaisit K. Sexual behavior assessment and semen analysis in young twin Brahman bulls (Bos indicus) born from in vitro produced bisected embryos. J Thai Vet Med Assoc.  50: 119-28, 1999.

International and National Conferences:

1.      Iamsaard, S., Saewu, A., Vanichviriyakit, R., Withyachumnarnkul, B. Basak, A., and Tanphaichitr, N. “Enzymatic activity of proprotein convertase 4 is important for mammalian fertilization”. 8th Annual OHRI Research Day, Nov 27, 2008, Hellenic Banquet Centre, Ottawa, ON, Canada.

2.      Iamsaard, S., Vanichviriyakit, R., Saewu, A., Withyachumnarnkul, B. Basak, A., and Tanphaichitr, N. “Enzymatic activity of proprotein convertase 4 is important for mammalian fertilization”. Biology of Reproduction: Fertilization (poster session B, No. 202). 41st SSR Annual Meeting, May 28, 2008, Kailua-Kona, Hawaii, USA.

3.      Iamsaard, S., Vanichviriyakit, R., Saewu, A., Withyachumnarnkul, B. Basak, A., and Tanphaichitr, N. “Enzymatic activity of proprotein convertase 4 is important for mammalian fertilization”. 26th Annual Ottawa Reproductive Biology Workshop, May 14, 2008, Norman Patterson Amphitheatre, the Ottawa Hospital Civic Campus, Ottawa, ON, Canada. (The Best Research Poster Presentation Award [Ph.D. Category]).

4.      Xu, H., Iamsaard, S., Kongmanas, K., Rupa T., and Tanphaichitr, N. “Significance of Arylsulfatase A in spermatogenesis and fertilization”. 26th Annual Ottawa Reproductive Biology Workshop, May 14, 2008, Norman Patterson Amphitheatre, the Ottawa Hospital Civic Campus, Ottawa, ON, Canada.

5.      Iamsaard, S., Vanichviriyakit, R., Saewu, A., Withyachumnarnkul, B. Basak, A., and Tanphaichitr, N. “Enzymatic activity of proprotein convertase 4 is important for mammalian fertilization”. 7th Annual OHRI Research Day, Nov 29, 2007, Hellenic Banquet Centre, Ottawa, ON, Canada.

6.      Iamsaard, S., Vanichviriyakit, R., Basak, A., and Tanphaichitr, N. “Enzymatic activity of sperm PC4 is important for mouse fertilization”. Second Sino-Canada Bilateral Workshop on Reproductive Health Research, October 19-21, 2007, Roger Guindon Hall, University of Ottawa, Ottawa, ON, Canada.

7.      Xu, H., Iamsaard, S., Kongmanas, K., Rupa, T., and Tanphaichitr, N. “Impairment of sperm fertilizing ability of arylsulfatase A (AS-A) knockout mice”. Biology of Reproduction: Fertilization and early embryogenesis (poster session D, No. 558). 40th SSR Annual Meeting, July 25, 2007, San Antonio, Texas, USA.

8.      Iamsard, S., Vanichviriyakit, R., Basak, A., and Tanphaichitr, N. “Proprotein convertase activity on the sperm surface is important for mouse fertilization”. Gordon Research Conference on Fertilization and Activation of Development, July 15-20, 2007, Holderness School, Plymouth, NH, USA.

9.      Xu, H., Iamsaard, S., Kongmanas, K., and Tanphaichitr, N. “Impairment of sperm fertilizing ability of arylsulfatase A (AS-A) knockout mice”. 25th Annual Ottawa Reproductive Biology Workshop, May 2, 2007, Hellenic Banquet Centre, Ottawa, ON, Canada.

10.    Iamsaard, S., Vanichviriyakit, R., Basak, A., and Tanphaichitr, N. “Roles of sperm surface PC4 activity in fertilization: Studies using a proPC4 peptide as the enzyme inhibitor”. 25th Annual Ottawa Reproductive Biology Workshop, May 2, 2007, Hellenic Banquet Centre, Ottawa, ON, Canada.

11.    Iamsaard, S., Vanichviriyakit, R., Basak, A., and Tanphaichitr, N. “Enzymatic activity of sperm PC4 is important for mouse fertilization”. 6th Annual OHRI Research Day, Nov 30, 2006, Hellenic Banquet Centre, Ottawa, ON, Canada.

12.    Iamsaard (Aiumsaard) S, Namking M, Buranarugsa M, Boonruangsri P,  Woraputtaporn W, Wongpanich V. Toxicological study on Coscinium fenestratum in rats. Poster presentation in JSPS-NRCT Core University System on Natural Medicine in Pharmaceutical Sciences The Joint Seminar; Recent Advances in Natural Medicine Research, Faculty of Pharmaceutical Science, Chulalongkorn University, Bangkok, Thailand, December 2-4, 2003.

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