Staff

 

Academic Staff

 

Vijittra Leardkamolkarn

Associate Professor
Department of Anatomy, Room B110
Faculty of Science, Mahidol University
Bangkok, 10400, Thailand

Telephone:
(Office)  +66 2201-5405
(Lab)     +66 201-5414
(Home)   +66 2964-5420
(Cellular) +66 8 1552-4797
Fax: +66 2354-7168
E-mail: scvlk@mahidol.ac.th

 

 

Postdoctoral training & Research experiences

1991-1992 Exchange Research Scholars (JSPS grant), Department of Internal Medicine, Nagoya University, Nagoya, JAPAN
1992-1993 Postdoctoral Fellow (Fogarty International/NIH grant), Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA
1993-1994 Postdoctoral Fellow (Fogarty International/NIH grant), Department of Cell Biology, University of Alabama at Birmingham , Birmingham, Alabama, USA
1995 Visiting Associate Professor, Department of Cell Biology, University of Alabama at Birmingham, USA (NIH grant)
1997-1998 Visiting Research Fellow, Molecular Virology Unit, Centers for Disease Control and Prevention, Department of Health and Human Service, National Institute of Health, Fort Collin, Colorado, USA

Appointments

1977-1981 Lecturer, Department of Anatomy, Mahidol University, THAILAND
1981-1985 Assistant Professor, Department of Anatomy, Mahidol University, THAILAND
1998-2001 Director of Graduate Studies, Department of Anatomy, Faculty of Science, Mahidol University, THAILAND
1990-present Associate Professor, Department of Anatomy, Mahidol University, THAILAND

Current Research Interests

Research activities in our laboratory involve 3 major studies:

1. Cell & Molecular Biology of Cancer:  I am interested in the roles of cell-cell and cell-substrate adhesion proteins involved in the process of tumor invasion and metastases. My current investigations are the studies on the role of a tumor associated antigen in immunopathogenesis of cancers, the role of intracellular molecules associated with cell-ECM interaction in mediation of tumor progression and the interplay between integrins and MMPs activation mechanism in cancers by using immunolocalization, molecular biology and cell culture approaches. In attempt to inhibit cancers, my research group is experimenting on anti-tumor formation, anti-tumor progression and metastasis agents using signaling inhibitors and natural extracts from plants. Additionally, we have currently set up in vitro models for studying and screening of cytotoxicity and genotoxicity effects of natural products used in industry and medicine.

2. Dengue vaccine development and anti-dengue drugs: My laboratory has constructed several mutants of 5NCR dengue virus from cDNA clone of dengue-2 (16681), using molecular genetic engineering approach. The derived viruses were characterized in cell culture, mouse and mosquito vector (Aedes Aegypti) and also evaluations of the mutants for RNA genetic stability, vertical transmission in vector, in vitro RNA transcription and protein translation. The goals of these studies are to reveal the molecular structural-functional relationship of Dengue 5NCR in viral replication and to improve dengue vaccine development. We have selected a few attenuated candidates that have high potential for vaccine to detail study on various aspects of the immune response and immunopathogenesis of dengue hemorrhagic fever (DF) and dengue shock syndrome (DHF/DSS). Recently, weve engineered the green fluorescent protein (GFP) gene into our dengue viruses to study viral activities in cells. Also, to establish stable cell lines that express dengue proteins with GFP reporter protein. This cell line is using as a high-throughput screening assay in searching for the anti-viral agents or inhibitors of dengue replication by medicinal plant extracts.

Publications

  1. Leardkamolkarn, V., and Sripairojthikoon W. 1981. Anomalies of the mylohyoid groove of the mandible. M. Dent. J. 2:85-90
  2. Sripairojthikoon W., and Leardkamolkarn, V 1982. Anatomical observation on the mylohyoid bridge: An anomalous derivative of Meckels cartilage, in the Thai. M. Dent. J. 3:76-85
  3. Visessuwan, S., Sriaroon U., Leardkamolkarn, V., and Supaluxanagorn M.. 1983. Roles of L-monosodium glutamate in sterility. Science (Thailand) 37:699-700.
  4. Sasaluxananon, S. Somana R., Leardkamolkarn, V., and W. Sripairojthikoon. 1983. Decrease in pituitary growth hormone level in rat after prolong administrations of bovine thyrotropin. Thai J. Pharmacol. 5:71-76
  5. Leardkamolkarn, V., R. Somana., S. Rojananuengnit, and P. Tosukhowong. 1986. Failure of inducing spermatogenesis with FSH, LH and testosterone in vitamin A deficient rat. Thai J. Pharmacol. 8:153-160
  6. Abrahamson, D.R., and Leardkamolkarn, V. 1985. Accelerated anti-laminin mediated anti-basement membrane disease. J. Cell Biol. 101:92a
  7. Leardkamolkarn, V., M.H. Irwin, W.F. Littlejohn, D.J. Salant and D.R. Abrahamson. 1987. Glomerular basement membrane (GBM) laminin distribution in nephrotoxic nephritis (NTN). J. Cell Biol. 105:42a
  8. Leardkamolkarn, V., and D.R. Abrahamson. 1988. Laminin biosynthesis in rat pituitary gonadotropic cells. J. Cell Biol. 107:588a
  9. Leardkamolkarn, V., and D.R. Abrahamson. 1988. Binding of intravenously injected antibodies against laminin to developing and mature endocrine glands. Cell Tissue Res. 251:171-181 (Impact Factor 2.383)
  10. Leardkamolkarn, V., and D.R. Abrahamson. 1989. Laminin localization in developing ovarian follicles. J. Cell Biol 109:237a
  11. Leardkamolkarn, V., L.W. Heck, and D.R. Abrahamson. 1989. Gonadectomy induces laminin biosynthesis and basement membrane assembly in anterior pituitary glands of adult rat. Cell Tissue Res. 257:587-596 (Impact Factor 2.383)
  12. Leardkamolkarn, V., D.J. Salant, and D.R. Abrahamson. 1990. Loss and rearrangement of glomerular basement membrane laminin during acute nephrotoxic nephritis in the rat. Am. J. Pathol. 137:187-198 (Impact Factor 5.796)
  13. Chinsethagij,K., B. Withyachumnarnkul, V. Leardkamolkarn, W. Trakulrungsi, P. Sobhon, and B. Poolsanguan. 1990. The sinus gland of the giant freshwater prawn, Macrobrachium rosenbergii de man, as revealed by scanning electron microscopy. J.E.M. Soc. Thailand 4:43-50
  14. Abrahamson, D.R., and V. Leardkamolkarn. 1991. Development of kidney tubular basement membranes. Kidney Int. 39:382-391(Impact Factor 4.927)
  15. Fogel, M.A., C.D. Boyd, V. Leardkamolkarn, D. R. Abrahamson, A W.M. Minto, and D.J. Salant. 1991. Glomerular basement membrane expansion in passive Heymann Nephritis. Absence of increased synthesis of type IV collagen, laminin, or fibronectin. Am. J. Pathol. 138:465-475 (Impact Factor 5.796)
  16. Chinsethagij, K., B. Withyachumnarnkul, B. Poolsanguan, V. Leardkamolkarn, and R.Somana. 1991. Major cell types in the optic lobe of the giant freshwater prawn, Macrobrachium rosenbergii de man. J.E.M. Soc. Thailand 5:9-23
  17. Leardkamolkarn, V., and D.R. Abrahamson. 1992. Immunoelectron microscopic-localization of laminin in rat ovarian follicles. Anat. Rec. 233:41-52 (a photograph in this article was printed on the Journals cover) (Impact Factor 1.444)
  18. Leardkamolkarn, V., and S. Matsuo. 1993. Alerations of mesangial specific protein during proliferative glomerulonephritis induced by administration of anti-thymocyte serum. J. Amer. Nephrol. 4(3) p. 657 (Impact Factor 7.505)
  19. Toskulkao, C., W. Deechakawan, V. Leardkamolkarn, and T. Glinsukon. 1994. The low calorie natural sweetener sterioside: Nephrotoxicity and its relationship to urinary enzyme excretion in the rat. Phytotherapy Res. 8:281-286 (Impact Factor 1.192)
  20. Van den Henval, G.B., P. St. John, V. Leardkamolkarn, and D.R. Abrahamson. 1994. Temporally and spatially regulated expression of rat kidney laminin B2. J. Amer. Nephrol. p.821 (Impact Factor 7.505)
  21. Leardkamolkarn, V. 1996 Excessive production of extracellular matrix limitted the formation of vesicles in polycystic ovarian (PCO) disease. J.E.M. Soc. Thailand 11(1)
  22. Van den Heuvel, G.B., V. Leardkamolkarn, P. St.John, and D.R. Abrahamson. 1996. Carboxy terminal sequence and synthesis of rat kidney laminin gamma-1 chain. Kidney Int. 49:752-760 (Impact Factor 4.927)
  23. Pinson, H. D., D.C. Tucker, P. St. John, V. Leardkamolkarn, M.A. Accavitti C.K. Abrass, and D.R. Abrahamson. 1996. Endogenous origin of glomerular endothelial and mesangial cells in grafts of embryonic kidneys. Amer J. Physiol. 270 (Renal Fluid Electrolyte Physiol. 39): F886-F899 (Impact Factor 4.263)
  24. Leardkamolkarn, V. 1997 Dynamic expression of Thy-1.1 antigen in the rat kidney. The XIVth International Congress of Nephrology. Sydney Australia. Nephrology (3) Supp.1, S79 (Impact Factor 1.215)
  25. Leardkamolkarn, V., N. Kurucharoen, and B. Withyachumnarnkul. 1997. Fine structure, development, and basement membrane components of coelomic sac of giant freshwater prawn, Macrobrachium rosenbergii de Man. J.E.M. Soc. Thailand 11(2) : 59-70
  26. Leardkamolkarn, V. 1998. Basement Membrane : Ultrastructure, Components and Immunolocalization. J.E.M. Soc. Thailand 12(1) : 37-49
  27. Nuntakarn, L., Meevatee, U., Witayachumnarnkul, B., Leardkamolkarn, V., and Fuchareon, S. 2002. Application of micro-FISH for characterization of structural human chromosome abnormalities. Science Asia 28:1-9
  28. V.Leardkamolkarn and S. Matsuo 2002 The glomerular reconstruction in acute mesangial glomerulonephritis is induced by specific Thy-1 epitopes on the mesangial cell surface. J.E.M. Soc. Thailand 16 (1): 91
  29. Leelawat, K., Watanabe, T., Nakajima, M., Tujinda, S., Suthipintawong, C., and Leardkamolkarn, V. 2003 Up regulation of tumor-associated antigen RCAS1 is implicated in high stages of colorectal cancer. J Clin Pathol 56(10):764-768 (Impact Factor 2.966, Journal Citation Reports, year 2003)
  30. Leelawat, K., Watanabe, T., Enjoji, M, Nakajima, M., Tujinda, S., Suthipintawong, C., and Leardkamolkarn, V. 2003. Increase serum level of RCAS1 antigen in cholangiocarcinoma patients correlates with tissue level of RCAS1 mRNA and protein expression. J Hepatol, 38:101-101 (Impact Factor 5.283, JCR year 2003)
  31. Leelawat K, Engprasert S, Pongchai-rerk U, Tujinda S, Suthipintawong C, and Leardkamolkarn V. 2004. No expression of Human Leukocyte Antigen G (HLA-G) in colorectal cancer cells. J Med Assoc Thai 87(7):816-818 (Scopus Citaton)
  32. Leelawat K, Engprasert S, Tujinda S, Suthipintawong C, Enjoji M, Nakashima, M, Watanabe T, and Leardkamolkarn V. 2005 Receptor-binding cancer antigen expressed on SiSo cells can be detected in metastatic lymph nodes from gastrointestinal cancers. World J Gastroenterology, 11(38): 6014-6017 (Scopus Citation)
  33. Pongchai-rerk U, Guan Jun-Lin, and Leardkamolkarn V. 2005 Focal adhesion kinase and Src phosphorylations in HGF-induced proliferation and invasion of human cholangiocarcinoma cell line, HuCCA-1. World J Gastroenterology, 11(37): 5845-5852 (Scopus Citation = 4, year 2007)
  34. Luplertlop N, Misse Dorothee, Bray Dorothy, Deleuze V, Gonzalez J-P, Leardkamolkarn V, Yssel H. and Veas F. 2006. Dengue-virus-infected dendritic cells trigger vascular leakage through metalloproteinase over production. European Molecular Biology Organization (EMBO reports), Nov. 7 (11);1176-1181 (Impact Factor 10.086, JCR year 2006)
  35. Borrirukwanit B, Lafleur MA, Mercuri FA. Blick T, Price JT, Fridman R, Pereira JJ, Leardkamonkarn V, and Thompson, EW. 2007. The type I collagen induction of MT1-MMP-mediated MMP-2 activation is repressed by aVß3 integrin in human breast cancer cells. Matrix Biol, 26:291-05 (Impact Factor 3.674, JCR year 2006)
  36. Sirigulpanit, W., Kinney, R.M., and Leardkamolkarn, V. 2007 Substitution or deletion mutations between nucleotides 55-69 in the 5 noncoding region of dengue type 2 virus produce variable effects on virus viability. J Gen Virol; 88:1748-1752 (Impact Factor 3.110, JCR year 2006)
  37. Kumkate,S., Onmek, N., Boonburapong, B., Singhakaew, S., and Leardkamolkarn, V. 2009 Estrogen-related fecundity reduction of lymnaea ollula following fasciola gigantica infection. J Parasitology (Jul 24:1 pubmed ahead). (Impact Factor 1.165)
  38. Leardkamolkarn V, Tiamyuyen S, and Sripanidkulchai B. 2009 Pharmacological activity of Kaempferia parviflora extract against human bile duct cancer cell lines. Asian Pacific Journal of Cancer Prevention; 10:695-698 (Scopus Citation)
    39. Leardkamolkarn V, Purimsuk, C, Himakoun, L, Kumkate, S, Sirigulpanit, W and Sripanidkulchai, BO 2009 Anti-Dengue Virus Effect of Houttuynia cordata Extract. (Submitted)
  39. Leardkamolkarn, V., Sirigulpanit, W., and Kinney, R.M. 2009 Characterization of recombinant dengue-2 virus derived from a single nucleotide substitution in the 5' non coding region. (Submitted)
  40. Sirigulpanit, W. and Leardkamolkarn, V. 2009 Construction and characterization of dengue-2 replicons containing 5'non-coding region substitution and green fluorescent protein replacement at the viral structural genes (Submitted)

Patent èԷԺѵ

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