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A. Flavin-dependent enzymes
A.1 A two-component enzyme ...
A.1 ... (cont.)
A.2 Pyranose oxidase
A.3 Bacterial Luciferase
B. Serine hydroxymethyl transferase
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Curriculum Vitae

Biography :
Name : Prof. Pimchai Chaiyen, Ph.D.
Birth : December 25, 1970, Phuket Province, Thailand
Address : Department of Biochemistry /
  Center for Excellence in Protein Structure and Function
  Faculty of Science, Mahidol University
  272 Rama VI Road, Bangkok 10400, THAILAND.
   
  Tel. 66 2-201-5607 (Office)
  Tel. 66 2-354-5596 (Dept. Lab), 66 2-354-5847 (CPSF Lab)
  Fax. 66 2-354-7174
  E-mail : pimchai.cha@mahidol.ac.th
  Website : http://www.sc.mahidol.ac.th/Chaiyen_P  

Education:
1992 B.Sc. (First Class Hons) Chemistry, Prince of Songkla University,Thailand
1997 Ph. D. Biological Chemistry, University of Michigan, Ann Arbor, U.S.A.
 
Research and Professional Experiences:
1993, 1996 Teaching assistant in Biochemistry Laboratory (BC 416/516) for undergraduate students and graduate students, University of Michigan, Ann Arbor.
1994 Teaching assistant in Enzyme Kinetics (BC 573) for graduate students, University of Michigan, Ann Arbor.
1997-2001 Lecturer, Department of Biochemistry, Faculty of Science, Mahidol University
2001-2005 Assistant Professor, Department of Biochemistry, Faculty of Science, Mahidol University
2005-2009 Associate Professor, Department of Biochemistry, Faculty of Science, Mahidol University
2008-2009 Deputy Chair, Department of Biochemistry, Faculty of Science, Mahidol University
2009-2011 Chair, Department of Biochemistry, Faculty of Science, Mahidol University
2009-present Professor, Department of Biochemistry, Faculty of Science, Mahidol University
2011-present Deputy Dean for Research, Faculty of Science, Mahidol University
 
Awards and Distinctions :
1985 Recipient of Distinguished Student Award from Princess Sirindhorn
1985-1997 Recipient of Scholarship from Development and Promotion of Science and Technology Talent Project
1993 Recipient of Dr.Tap Nilaniti Outstanding Graduate Award
1995 Recipient of Chrisman Award from Department of Biological Chemistry, University of Michigan, Ann Arbor. (for outstanding Ph.D. candidate)
1998 Recipient of Murphy Award from Department of Biological Chemistry, University of Michigan, Ann Arbor. (for the outstanding publication series)
2000 Recipient of Federation of Asian and Oceanic Biochemistry and Molecular Biology (FAOBMB) travel fellowship
2003 Recipient of the L'oreal-Unesco Fellowship for Woman in Science in Thailand
2005 Young Scientist Award, Foundation for the Promotion of Science and Technology under the Patronage of H. M. the King, Thailand.
2008 Affiliate Fellow of TWAS (The Academy of Sciences for the Developing World)
2009 BMB Award from Section of Biochemistry and Molecular Biology, The Science Society of Thailand under the Patronage of His Majesty the King.
2009 Faculty of Science Outstanding Lecturer Award (First-Middle Level)
2010 Exemplary Lecturer Award from Mahidol University Faculty Senate
2010 Taguchi Prize for Outstanding Research Achievement in Biotechnology
2010 TRF-CHE-Scopus Researcher Award 2010
2011 Outstanding Alumni Award 2011 from Prince of Songkla University
 
Ad Hoc Journal Reviewer :
  Acta Crystallographica Section F
  Applied Microbiology and Biotechnology
  Archives of Biochemistry and Biophysics
 

Advanced Synthesis & Catalysis

  Biochemistry
  Biochimica Biophysica Acta
  Bioresource Technology
  Chemistry & Biology
 

FEMS Microbiology Letter

  Journal of Agricultural and Food Chemistry
  Journal of Chemical Education
 

Journal of the American Chemical Society

 

The Journal of Biological Chemistry (JBC)

   
Reviewer of Funding Organizations :
  Austrian Science Fund
 

National Science Foundation (NSF, USA.)

  The Thailand Research Fund (TRF)
  National Science and Technology Development Agency (NSTDA)
  Commission on Higher Education (CHE), Ministry of Education
  Mahidol University
   
Field of Interest :
  Enzymology; Enzymes
Enzyme catalysis; Enzyme mechanism
Flavin and Flavoproteins
Enzyme Kinetics; Pre-steady state kinetics
Redox Enzymes; pyridoxal-5-phosphate
Physical Biochemistry; Biosensor

 
Publications :
Journal Articles
1997
1. Chaiyen P, Brissette P, Ballou DP, Massey V. Reaction of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase with N-methyl-5-hydroxynicotinic acid: Studies on the mode of binding, and protonation status of the substrate. Biochemistry 1997;36(45):13856-64.
   
2. Chaiyen P, Ballou DP, Massey V. Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase. Proceedings of the National Academy of Sciences of the United States of America 1997;94(14):7233-8.
   
3. Chaiyen P, Brissette P, Ballou DP, Massey V. Unusual mechanism of oxygen atom transfer and product rearrangement in the catalytic reaction of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase. Biochemistry 1997;36(26):8060-70.
   
4. Chaiyen P, Brissette P, Ballou DP, Massey V. Thermodynamics and reduction kinetics properties of 2-methyl-3- hydroxypyridine-5-carboxylic acid oxygenase. Biochemistry 1997;36(9):2612-21.
   
2001
5. Chaiyen P, Suadee C, Wilairat P. A novel two-protein component flavoprotein hydroxylase p-hydroxyphenylacetate hydroxylase from Acinetobacter baumannii. European Journal of Biochemistry 2001;268(21):5550-61.
   
2004
6. Chaiyen P, Sucharitakul J, Svasti J, Entsch B, Massey V, Ballou DP. Use of 8-Substituted-FAD Analogues to Investigate the Hydroxylation Mechanism of the Flavoprotein 2-Methyl-3-hydroxypyridine-5-carboxylic Acid Oxygenase. Biochemistry 2004;43(13):3933-43.
   
7. Thotsaporn K, Sucharitakul J, Wongratana J, Suadee C, Chaiyen P. Cloning and expression of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii: Evidence of the divergence of enzymes in the class of two-protein component aromatic hydroxylases. Biochimica et Biophysica Acta - Gene Structure and Expression 2004;1680(1):60-6.
   
2005
8. Hommalai G, Chaiyen P, Svasti J. Studies on the transglucosylation reactions of cassava and Thai rosewood b-glucosidases using 2-deoxy-2-fluoro-glycosyl-enzyme intermediates. Archives of Biochemistry and Biophysics 2005;442(1):11-20.
   
9. Oonanant W, Sucharitakul J, Yuvaniyama J, Chaiyen P. Crystallization and preliminary X-ray crystallographic analysis of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase from Pseudomonas sp. MA-1. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 2005;61(3):312-4.
   
10. Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. The reductase of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii requires p-hydroxyphenylacetate for effective catalysis. Biochemistry 2005;44(30):10434-42.
   
2006
11. Kujawa M, Ebner H, Leitner C, Hallberg BM, Prongjit M, Sucharitakul J, Ludwig R, Rudsander U, Peterbauer C, Chaiyen P, Haltrich D, Divne C. Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase. Journal of Biological Chemistry 2006;281(46):35104-15.
   
12. Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. Kinetic mechanisms of the oxygenase from a two-component enzyme, p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii. Journal of Biological Chemistry 2006;281(25):17044-53.
   
2007
13. Alfieri A, Fersini F, Ruangchan N, Prongjit M, Chaiyen P, Mattevi A. Structure of the monooxygenase component of a two-component flavoprotein monooxygenase. Proceedings of the National Academy of Sciences of the United States of America 2007;104(4):1177-82.
   
14. Suadee C, Nijvipakul S, Svasti J, Entsch B, Ballou DP, Chaiyen P. Luciferase from Vibrio campbellii is more thermostable and binds reduced FMN better than its homologues. Journal of Biochemistry 2007;142(4):539-52.
   
15. Sucharitakul J, Phongsak T, Entsch B, Svasti J, Chaiyen P, Ballou DP. Kinetics of a two-component p-hydroxyphenylacetate hydroxylase explain how reduced flavin is transferred from the reductase to the oxygenase. Biochemistry 2007;46(29):8611-23.
   
2008
16. Nijvipakul S, Wongratana J, Suadee C, Entsch B, Ballou DP, Chaiyen P. LuxG is a functioning flavin reductase for bacterial luminescence. Journal of Bacteriology 2008;190(5):1531-8.
   
17. Sucharitakul J, Prongjit M, Haltrich D, Chaiyen P. Detection of a C4a-Hydroperoxyflavin Intermediate in the Reaction of a Flavoprotein Oxidase. Biochemistry 2008;47(33):8485–90.
   
2009
18. Spadiut O, Pisanelli I, Maischberger T, Peterbauer C, Gorton L, Chaiyen P, Haltrich D. Engineering of pyyranose 2-oxidase: improvement for biofuel cell and food applications through semi-rational protein design. Journal of Biotechnology 2009; 139(3):250-7.
   
19. Prongjit M, Sucharitakul J, Wongnate T, Haltrich D, Chaiyen P. Kinetic mechanism of pyranose 2-oxidase from Trametes multicolor. Biochemistry 2009;48(19):4170-80.
   
20. Chosrowjan H, Taniguchi S, Mataga N, Phongsak T, Sucharitakul J, Chaiyen P, Tanaka F. Ultrafast Solvation Dynamics of flavin mononucleotide in the reductase component of p-Hydroxyphenylacetate Hydroxylase. Journal of Physical Chemistry B 2009;113(25):8439-42.
   
21. Baron R, Riley C, Chenprakhon P, Thotsaporn K, Winter RT, Alfieri A, Forneris F, van Berkel WJH, Chaiyen P, Fraaije MW, Mattevi A, McCammon JA. Multiple pathways guide oxygen diffusion into flavoenzyme active sites. Proceedings of the National Academy of Sciences of the United States of America 2009;106(25):10603-8.
  [ Cited in "Research Highlights" Nature Chemistry, 2009]
   
22. Sopitthammakhun K, Maenpuen S, Yuthavong Y, Leartsakulpanich U, Chaiyen P. Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues. FEBS Journal 2009;276(15):4023-36.
   
23. Maenpuen S, Sopitthammakhun K, Yuthavong Y, Chaiyen P, Leartsakulpanich U. Characterization of Plasmodium falciparum serine hydroxymethyltransferase—A potential antimalarial target. Molecular and Biochemical Parasitology 2009;168(1):63-73.
   
2010
24. Chaiyen P. Flavoenzymes catalyzing oxidative aromatic ring-cleavage reactions. Arch Biochem Biophys. 2010;493(1):62-70. (Review)
   
25. Pitsawong W, Sucharitakul J, Prongjit M, Tan TC, Spadiut O, Haltrich D, Divne C, Chaiyen P. A conserved active-site threonine is important for both sugar and flavin oxidations of pyranose 2-oxidase. Journal of Biological Chemistry 2010;285(13):9697-705.
   
26. Sucharitakul J, Wongnate T, Chaiyen P. Kinetic isotope effects on the noncovalent flavin mutant protein of pyranose 2-oxidase reveal insights into the flavin reduction mechanism. Biochemistry 2010;49(17):3753-65.
   
27. Chenprakhon P, Sucharitakul J, Panijpan B, Chaiyen P. Measuring binding affinity of protein. Ligand interaction using spectrophotometry: binding of neutral red to riboflavin-binding protein. Journal Chemical Education 2010;87(8):829-31.
   
28. Rujkorakarn R, Nunthaboot N, Tanaka F, Chaiyen P, Chosrowjan H, Taniguchi S, Mataga N. Time-resolved Stokes shift in proteins with continuum model: Slow dynamics in proteins. Journal of Photochemistry and Photobiology A: Chemistry 2010;215(1):38-45.
   
29. Tan TC, Pitsawong W, WongnateT, Spadiut O, Haltrich D, Chaiyen P, Divne C. H-bonding and Positive Charge at the N(5)/O(4) Locus are Critical for Covalent Flavin Attachment in Trametes Pyranose 2-Oxidase. Journal of Molecular Biology 2010;402(3):578-94
   
30. Nijvipakul S, Ballou SP, Chaiyen P. Reduction Kinetics of a Flavin Oxidoreductase LuxG from Photobacterium leiognathi (TH1):Half Sites Reactivity. Biochemistry 2010;49 (43):9241–8
   
2011
31. Ruangchan N, Tongsook C, Sucharitakul J, Chaiyen P. pH-dependent studies reveal an efficient hydroxylation mechanism of the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase. Journal of Biological Chemistry 2011;286(1):223-33.
   
32. Sucharitakul J, Wongnate T, Chaiyen P. Hydrogen peroxide elimination from C4A-hydroperoxy-flavin in a flavoprotein oxidase occurs through a single proton transfer from flavin N5 to a peroxide leaving group. Journal of Biological Chemistry 2011;286(19):16900-9.
   
33. Thotsaporn K, Chenprakhon P, Sucharitakul J, Mattevi A, Chaiyen P. Stabilization of C4A-hydroperoxy-flavin in a two-component flavin-dependent monooxygenase is achieved through interactions at flavin N5 and C4a atoms. Journal of Biological Chemistry 2011;286(32):28170-80.
   
34.

Penpassakarn P, Chaiyen P, Palittapongarnpim P. Efficient heterologous expression and one-step purification of fully active c-terminal histidine-tagged uridine monophosphate kinase from Mycobacterium tuberculosis. The Southeast Asian Journal of Tropical Medicine and Public Health 2011;42(6):1452-1459.

   
35.

Wongnate T, Sucharitakul J , and Chaiyen P. Identification of a catalytic base for sugar oxidation in pyranose 2-oxidase reaction. Chembiochem 2011;12(17):2577-86.

   
36.

Tongsook C, Sucharitakul J, Thotsaporn K, Chaiyen P. Interactions with the substrate phenolic group are essential for hydroxylation by the oxygenase component of p-hydroxyphenylacetate 3-hydroxylase. Journal of Biological Chemistry 2011;286(52):44491–502.

   
2012
   
37.

Chenprakhon P, Panijpan B, Chaiyen P. An Experiment Illustrating the Change in Ligand pKa upon Protein Binding. Journal of Chemical Education 2012, in press.

   
38.

Chosrowjan H, Taniguchi S, Wongnate T, Sucharitakul J, Chaiyen P, Tanaka F. Conformational heterogeneity in pyranose 2-oxidase from Trametes multicolor revealed by ultrafast fluorescence dynamics. Journal of Photochemistry and Photobiology A: Chemistry 2012;234(April 15):44-48.

   
39.

Oonnanat W, Sucharitakul J, Chaiyen P, Yuaniyama J. Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii. Acta Crystallographica Section F 2012, in press.

   
40.

Sucharitakul J, Wongnate T, Montersino S, van Berkel, WJH, Chaiyen P. Reduction Kinetics of 3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii RHA1. Biochemistry 2012. Just Accepted.

   
41.

Somprasong N, Jittawuttipoka T, Duang-nkern J, Romsang, A, Chaiyen P, Schweizer H, Vattanaviboon P, Mongkolsuk S. Pseudomonas aeruginosa thiol peroxidase protects against hydrogen peroxide toxicity and displays atypical patterns of gene regulation. Journal of Bacteriology 2012. Just Accepted.

 
Chapter in Book (Annual Report)
1.

Chaiyen P, Suadee C, Meevoothisom V. Degradation of phenylacetic acid by bacteria from Thai soil. In: Annual Report of ICBiotech. Vol. 21. Osaka: International Center for Biotechnology; 1998. p. 862-7.

 
Papers in Proceedings
1.

Chaiyen P, Suadee C, Thotsaporn K, Svasti J. Studies of the two-component p-hydroxyphenylacetate hydroxylase from Acinetobacter baumannii. In: Chapman S, Perham R, Scrutton N, editors. Flavins and Flavoproteins 2002: Proceedings of the Fourteenth International Symposium; 2002 July 14-18; St. John's College, University of Cambridge, Uk. Berlin: Agency for Scientific Publications; 2002. p. 975-80.

 

2.

Sucharitakul J, Chaiyen P, Ballou DP, Massey V. Probing the mechanism of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase by using 8-substituted-FAD analogs. In: Chapman S, Perham R, Scrutton N, editors. Flavins and Flavoproteins 2002: Proceedings of the Fourteenth International Symposium; 2002 July 14-18; St. John's College, University of Cambridge, Uk. Berlin: Agency for Scientific Publications; 2002. p. 381-6.

 

3.

Sucharitakul J, Chaiyen P, Entsch B, Ballou DP. Mechanistic studies on the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii.. In: Nishino T, Miura R, Tanokura M, Fukui K, editors. Flavins and Flavoproteins 2005: Proceedings of the Fifteenth International Symposium; 2005 April 17-22; Shonan Village Center, Hayama, Japan: ARchiTect Inc.; 2005. p. 275-80.

 

4.

Chaiyen P, Phongsak T, Sucharitakul J, Ballou DP. Review and perspectives of how reduced flavin is transferred between the reductase and oxygenase p-hydroxyphenylacetate 3-hydroxylase. In: Frago S, Gomez-moreno C, Medina M, editors. Flavins and Flavoproteins 2008: Proceedings of the Sixteenth International Symposium; 2008 June 8-13; Jaca, Spain: Prensas Universitarias de Zaragoza; 2008. p. 27-44.

 
Oral Presentations (International Level)
1.
Oral Presentation
“p-Hydroxyphenylacetic acid hydroxylase from Acinetobacter baumannii” 15th the Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB) Symposium, Beijing, China, October 21-24, 2001.
   
2.
Invited Seminar Lecture
"Reduced Flavin Transfer between Components of p-Hydroxyphenyl- acetate 3-Hydroxylase". A seminar at University of Pavia, Italy, June 12, 2006.
   
3.
Invited Symposium Lecture
"How the reductase and oxygenase work together in a two-component flavoenzyme, p-hydroxyphenylacetate". 16th International Symposium on Flavins and Flavoproteins, Jaca, Spain , Jun 10, 2008.
   
4. Invited Speaker
Gordon Research Conference 2010: Enzymes, Co-enzymes & Metabolic Pathways "What is necessary for C4a-hydroperoxy-FAD formation in pyranose 2-oxidase reacion?" July 19-23, 2010 at Waterville Valley Resort, New Hampshire, USA.
   
5. Invited seminar lecture
" Exploring the Reaction Mechanism of Pyranose 2-Oxidase Through Transient Kinetics and Site-Directed Mutagenesis" at Wake Forest University, July 27, 2010, Winston-Salem, North Carolina, USA.
   
6. Invited Symposium lecture
“Oxygenation mechanism of p-hydroxyphenylacetate hydroxylase, a two-component flavin-dependent monooxygenase”. The 3rd Asia Pacific Protein Association (APPA) Conference, May 6-9, Shanghai, China.
   
7. Invited Symposium lecture
“Use of Kinetic Isotope Effects and Transient Kinetics to Unravel the Mechanism of H2O2 Elimination from C4a-Hydroperoxy-flavin in Pyranose2-Oxidase”. The IXth European Symposium of The Protein Society. May 22-26, 2011, Stockholm, Sweden.
   
8. Invited Symposium lecture
“Understanding reaction mechanisms of two-component flavin-dependent monooxygenases through a model of p-hydroxyphenylacetate hydroxylase”. 17th International Conference on Cytochrome P450, at The University of Manchester, UK 26th to 30th of June, 2011.
   
   
9. Oral Presentation
Mechanistic Studies of Flavin- and PLP-Dependent Enzymes. Presentation by TWAS young affiliate at 22nd General Meeting at International centre in Theroretical Physics, Trieste, Italy, on 21-23 November 2011.
   
 
Invited Symposium Lectures in Thailand
1.
Chaiyen P, Sucharitakul J, Thotsaporn K, Suadee C, Entsch B, Ballou DP. 2005. Two-component flavoproteins in oxygenation reactions. The 2nd Protein Research Network Symposium on Proteins Structure, Function, and Proteomics, Chulabhorn Research Institute Conference Center, Bangkok, Thailand.
   
2.
Chaiyen C, Sucharitakul J, Suadee C, Thotsaporn K, Entsch B, Ballou DP. 2005. Reaction Mechanism of p-Hydroxyphenylacetate Hydroxylase. The 31th Congress on Science and Technology of Thailand, Nakhon Ratchasima, Thailand.
   
3.
Chaiyen P, Nijvipakul S, Suadee C. 2006. A Novel Luciferase From Vibrio Cambellii : Implication on residues important for reduced FMN binding. Annual Conference of Thai Society for Biotechnology, Bangkok, Thailand.
   
4.
Chaiyen P, Sucharitakul J, Phongsak T, Entsch B, Ballou DP. 2006. Reduced Flavin Transfer between components of p-hydroxyphenylacetate hydroxylase. First Annual Symposium of the Protein Society of Thailand, October 24-25, Bangkok, Thailand.
   
5.
Chaiyen P. 2009. Use of Pre-Steady State Kinetics to Elucidate Reaction Mechanisms of Flavin-Dependent Enzymes. The 2nd Biochemistry and Molecular Biology (BMB) Conference 2009: Biochemistry and Molecular Biology for Regional-Sustainable Development, May 7-8, 2009, Khonkaen.
   
6.
Chaiyen P. 2009. Plasmodium serine hydroxymethyl transferase as an antimalarial target. Fourth Annual Symposium Protein Society of Thailand Protein Research: from Basic Studies to Applications in Health Sciences, Aug 21, 2009, Chulabhorn Research Institute, Bangkok, Thailand.
   
7.
Chaiyen P. 2010. Pyranose 2-Oxidase: Kinetics and Mechanisms. PACCON 2010 Pure and Applied Chemistry Conference 2010, Jan 21-23, 2010, Sunee Grand Hotel and Convention Center, Ubonratchathani, Thailand.
   
8.
Chaiyen P. 2010. The Quest to Explore How Enzymes Work. TRF-CHE-Scopus Researcher Award Lecture at the 10th Thailand Research Fund Annual Meeting, 14-16 October 2010, at Holiday Inn Resort Regent Beach, Cha-am, Petchaburi, Thailand.
   
9.
Chaiyen P. 2010. Mechanistic Studies of Flavin-Dependent and PLP-Dependent Enzymes. Taguchi Prize for Outstanding Research Achievement in Biotechnology 2010 Lecture at International Conference on Biotechnology for Healthy Living. The 22th Annual Meeting of the Thai Society for Biotechnology 20-22 October 2010, at Prince Songkla University, Trang Campus, Thailand.
   
10.
Chaiyen P. 2011. Exploring the Reaction Mechanism of Pyranose 2-Oxidase Through Transient Kinetics. At the 4rd International Conference for Value Added Agricultural Products on August 29-31, 2011 in Khon Kaen, Thailand.
   
11.
Chaiyen P. 2011. Understanding the Oxygenation Mechanism of Flavin-Dependent Monooxygenases through a Model of p-Hydroxyphenylacetate Hydroxylase. The 37th Congress on Science and technology of Thailand (STT37), October 12, 2011 at Centara Grand & Bangkok Convention Centre at Central World, Bangkok, Thailand.


 

 

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